Neurotrophic factor effects on oxidative stress-induced neuronal death.
Neurotrophic factor effects on oxidative stress-induced neuronal death.
复制标题
神经营养因子对氧化应激诱导的神经元死亡的影响。
DOI：
10.1023/a:1022817918651
复制
发表时间：
2003
影响因子：
4.4
通讯作者：
Hjelmhaug,Julie
中科院分区：
文献类型：
--
作者：
Lobner,Doug;Golner,Susan;Hjelmhaug,Julie
神经营养因子已被证明在皮层培养物中增强坏死性神经元死亡。在这项研究中，我们的特点是各种氧化损伤诱导的死亡，并测试了神经营养因子对死亡的影响。用成纤维细胞生长因子-2、神经营养因子-4或胰岛素样生长因子-1处理可增强由柠檬酸铁（Fe）或丁硫氨酸亚砜亚胺（BSO）诱导的神经元细胞死亡，但不能增强依他尼酸（EA）。自由基清除剂trolox可阻断每次损伤引起的神经元死亡。死亡的分析表明，铁和BSO诱导坏死细胞死亡，而EA诱导凋亡细胞死亡。BSO和EA引起细胞谷胱甘肽水平下降，而Fe对谷胱甘肽水平没有影响。神经营养因子对谷胱甘肽的变化无影响。结果表明，氧化损伤可诱导细胞凋亡或坏死性死亡，神经营养因子的作用依赖于细胞死亡的类型。
Neurotrophic factors have been shown to potentiate necrotic neuronal death in cortical cultures. In this study we characterized the death induced by various oxidative insults and tested the effects of neurotrophic factors on that death. Treatment with fibroblast growth factor-2, neurotrophin-4, or insulin-like growth factor-1 potentiated neuronal cell death induced by iron-citrate (Fe) or buthionine sulfoximine (BSO), but not ethacrynic acid (EA). Neuronal death induced by each insult was blocked by the free radical scavenger, trolox. An analysis of the death indicated that Fe and BSO induced necrotic cell death, while EA induced apoptotic cell death. BSO and EA caused decreased cellular glutathione levels, whereas Fe had no effect on glutathione levels. Neurotrophic factors had no effect on the changes in glutathione. The results indicate that oxidative insults can induce either apoptotic or necrotic death and that the effects of neurotrophic factors are dependent on the type of cell death.