HIV-1 DNA sequence diversity and evolution during acute subtype C infection
HIV-1 DNA sequence diversity and evolution during acute subtype C infection
复制标题
急性C亚型感染期间HIV-1 DNA序列多样性和进化
DOI：
10.1038/s41467-019-10659-2
复制
发表时间：
2019-06-21
影响因子：
16.6
通讯作者：
Lichterfeld, Mathias
中科院分区：
文献类型：
--
作者：
Lee, Guinevere Q.;Reddy, Kavidha;Lichterfeld, Mathias
Little is known about the genotypic make-up of HIV-1 DNA genomes during the earliest stages of HIV-1 infection. Here, we use near-full-length, single genome next-generation sequencing to longitudinally genotype and quantify subtype C HIV-1 DNA in four women identified during acute HIV-1 infection in Durban, South Africa, through twice-weekly screening of high-risk participants. In contrast to chronically HIV-1-infected patients, we found that at the earliest phases of infection in these four participants, the majority of viral DNA genomes are intact, lack APOBEC-3G/F-associated hypermutations, have limited genome truncations, and over one year show little indication of cytotoxic T cell-driven immune selections. Viral sequence divergence during acute infection is predominantly fueled by single-base substitutions and is limited by treatment initiation during the earliest stages of disease. Our observations provide rare longitudinal insights of HIV-1 DNA sequence profiles during the first year of infection to inform future HIV cure research.
