Autoantibodies Against Proteins Previously Associated With Autoimmunity in Adult and Pediatric Patients With COVID-19 and Children With MIS-C.
Autoantibodies Against Proteins Previously Associated With Autoimmunity in Adult and Pediatric Patients With COVID-19 and Children With MIS-C.
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DOI：
10.3389/fimmu.2022.841126
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发表时间：
2022
影响因子：
7.3
通讯作者：
Cohen JI
中科院分区：
文献类型：
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作者：
Burbelo PD;Castagnoli R;Shimizu C;Delmonte OM;Dobbs K;Discepolo V;Lo Vecchio A;Guarino A;Licciardi F;Ramenghi U;Rey-Jurado E;Vial C;Marseglia GL;Licari A;Montagna D;Rossi C;Montealegre Sanchez GA;Barron K;Warner BM;Chiorini JA;Espinosa Y;Noguera L;Dropulic L;Truong M;Gerstbacher D;Mató S;Kanegaye J;Tremoulet AH;Pediatric Emergency Medicine Kawasaki Group;Eisenstein EM;Su HC;Imberti L;Poli MC;Burns JC;Notarangelo LD;Cohen JI
新冠肺炎或儿童多系统炎症综合征患者中先前与自身免疫性疾病和其他人类蛋白相关的自身抗原的抗体谱仍不明确。在这里，我们发现30%患有新冠肺炎的成年人有针对肺抗原KCNRG的自身抗体，34%有针对系统性红斑狼疮相关的Smith-D3蛋白的抗体。患有新冠肺炎的儿童很少出现自身抗体；59名儿童中有一名患有与胰岛素依赖型糖尿病急性发作有关的GAD65自身抗体。虽然74%(40/54)的MIS-C患者检测到与SLE/Sjögren综合征(Ro52、Ro60和La)和/或自身免疫性胃炎(胃ATPase)相关的自身抗体，但对这些患者和川崎病(KD)儿童的进一步分析表明，静脉注射免疫球蛋白(IVIG)在两组患者中检测这些自身抗体的主要原因是静脉注射免疫球蛋白(IVIG)。在体内监测患者体内自身抗体的衰变情况显示，IVIG衍生的Ro52、Ro60和La自身抗体在45-60天后下降到无法检测到的水平，但胃ATPase自身抗体下降得更慢，需要100天才能检测到。在未接受静脉注射免疫球蛋白的患者中，针对已发表的对MIS-C的自身抗原阵列研究确定的潜在靶点的子集的进一步测试未能检测到针对大多数这些蛋白质的自身抗体(16/18)。而肌钙蛋白C2和KLHL12自身抗体的阳性率分别为2/20和1/20。总体而言，这些结果表明，IVIG治疗可能是测定MIS-C中自身抗体的一个混杂因素，而抗自身免疫性疾病相关抗原或其他人类蛋白的抗体在MIS-C中并不常见。
The antibody profile against autoantigens previously associated with autoimmune diseases and other human proteins in patients with COVID-19 or multisystem inflammatory syndrome in children (MIS-C) remains poorly defined. Here we show that 30% of adults with COVID-19 had autoantibodies against the lung antigen KCNRG, and 34% had antibodies to the SLE-associated Smith-D3 protein. Children with COVID-19 rarely had autoantibodies; one of 59 children had GAD65 autoantibodies associated with acute onset of insulin-dependent diabetes. While autoantibodies associated with SLE/Sjögren’s syndrome (Ro52, Ro60, and La) and/or autoimmune gastritis (gastric ATPase) were detected in 74% (40/54) of MIS-C patients, further analysis of these patients and of children with Kawasaki disease (KD), showed that the administration of intravenous immunoglobulin (IVIG) was largely responsible for detection of these autoantibodies in both groups of patients. Monitoring in vivo decay of the autoantibodies in MIS-C children showed that the IVIG-derived Ro52, Ro60, and La autoantibodies declined to undetectable levels by 45-60 days, but gastric ATPase autoantibodies declined more slowly requiring >100 days until undetectable. Further testing of IgG and/or IgA antibodies against a subset of potential targets identified by published autoantigen array studies of MIS-C failed to detect autoantibodies against most (16/18) of these proteins in patients with MIS-C who had not received IVIG. However, Troponin C2 and KLHL12 autoantibodies were detected in 2 of 20 and 1 of 20 patients with MIS-C, respectively. Overall, these results suggest that IVIG therapy may be a confounding factor in autoantibody measurements in MIS-C and that antibodies against antigens associated with autoimmune diseases or other human proteins are uncommon in MIS-C.
影响因子：
3.7
作者：
Burbelo PD;Lebovitz EE;Bren KE;Bayat A;Paviol S;Wenzlau JM;Barriga KJ;Rewers M;Harlan DM;Iadarola MJ
通讯作者：
Iadarola MJ