Endothelin-induced conversion of embryonic heart muscle cells into impulse-conducting Purkinje fibers
Endothelin-induced conversion of embryonic heart muscle cells into impulse-conducting Purkinje fibers
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DOI：
10.1073/pnas.95.12.6815
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发表时间：
1998-06-09
影响因子：
11.1
通讯作者：
Mikawa, T
中科院分区：
文献类型：
--
作者：
Gourdie, RG;Wei, Y;Mikawa, T
有规律的心跳依赖于专门的起搏和传导细胞网络。关于这些心脏组织的发育起源一直存在长期争议，这些心脏组织也表现出神经样特性。最近，我们已经得出结论，在鸡胚胎发育过程中，脉冲传导浦肯野细胞从与冠状动脉发育空间相关的心肌细胞中招募。在这里，我们报告，培养的胚胎心肌细胞转化为浦肯野细胞表型暴露后，血管细胞因子，内皮素。这种诱导反应在发育过程中逐渐下降。这些结果进一步证明了动脉形成在诱导心肌谱系内的脉冲传导浦肯野细胞中的作用，并且也可以为心脏起搏和传导细胞的组织工程提供基础。
A regular heart beat is dependent on a specialized network of pacemaking and conductive cells. There has been a longstanding controversy regarding the developmental origin of these cardiac tissues which also manifest neural-like properties. Recently, we have shown conclusively that during chicken embryogenesis, impulse-conducting Purkinje cells are recruited from myocytes in spatial association with developing coronary arteries. Here, we report that cultured embryonic myocytes convert to a Purkinje cell phenotype after exposure to the vascular cytokine, endothelin. This inductive response declined gradually during development. These results yield further evidence for a role of arteriogenesis in the induction of impulse-conducting Purkinje cells within the heart muscle lineage and also may provide a basis for tissue engineering of cardiac pacemaking and conductive cells.