Biomolecule detection via target mediated nanoparticle aggregation and dielectrophoretic impedance measurement
Biomolecule detection via target mediated nanoparticle aggregation and dielectrophoretic impedance measurement
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DOI：
10.1039/b417535b
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发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Smith, RL
中科院分区：
文献类型：
--
作者：
Costanzo, PJ;Liang, EZ;Smith, RL
描述了一种新的生物传感系统，该系统基于目标生物分子对纳米颗粒的聚集以及这些聚集体的介电泳阻抗测量。通过荧光显微镜在微通道内验证了聚集过程，证明该过程可用于实时传感器应用。采用正介电泳来捕获薄膜电极边缘的纳米颗粒聚集体，通过荧光显微镜的光学成像或通过测量相邻电极之间的电阻抗变化来检测它们的存在。电检测机制证明了该方法作为微全分析系统（mTAS）的潜力。
A new biosensing system is described that is based on the aggregation of nanoparticles by a target biological molecule and dielectrophoretic impedance measurement of these aggregates. The aggregation process was verified within a microchannel via fluorescence microscopy, demonstrating that this process can be used in a real time sensor application. Positive dielectrophoresis is employed to capture the nanoparticle aggregates at the edge of thin film electrodes, where their presence is detected either by optical imaging via fluorescence microscopy or by measuring the change in electrical impedance between adjacent electrodes. The electrical detection mechanism demonstrates the potential for this method as a micro total analysis system (mTAS).