The Study on the Relationship Between IRS-1 Gly972Arg and IRS-2 Gly1057Asp Polymorphisms and Type 2 Diabetes in the Kurdish Ethnic Group in West Iran
The Study on the Relationship Between IRS-1 Gly972Arg and IRS-2 Gly1057Asp Polymorphisms and Type 2 Diabetes in the Kurdish Ethnic Group in West Iran
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DOI：
10.1089/gtmb.2012.0160
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发表时间：
2012-11-01
影响因子：
1.4
通讯作者：
Bakhtiyari, Salar
中科院分区：
文献类型：
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作者：
Haghani, Karimeh;Bakhtiyari, Salar
IRS-1 Gly 972 Arg和IRS-2 Gly 1057 Asp多态性与不同种族2型糖尿病（T2 DM）的相关性存在争议。我们的目的是确定这些多态性与伊朗库尔德族T2 DM的关联。研究组包括336例T2 DM和341例血糖正常受试者。基因分型采用聚合酶链反应-限制性片段长度多态性分析。然后评估基因型和等位基因频率。IRS-1Gly 972 Arg变异体的GR和RR基因型具有更高的T2 DM风险（比值比[OR]分别为1.76和3.86）。IRS-1Gly 972 Arg多态性与T2 DM的显性模型（GG vs. GR + RR）显著相关（OR = 1.63）。IRS-2 Gly 1057 Asp突变体的GD基因型与T2 DM的发病风险相关（OR = 1.63）。IRS-2 Gly 1057 Asp基因型（GG vs. GD + DD）的显性模型分析也显示与T2 DM的关联增强（OR = 1.69）。在几种组合中，GR/GD的T2 DM风险最高（OR = 3.1）。其他组合也与T2 DM显著相关，包括GR/GG（OR = 1.86）、RR/GG（OR = 1.76）、GG/GD（OR = 1.83）和GG/DD（OR = 2.35）。与GG基因型相比，GR + RR基因型对照组的HbA 1c、血清甘油三酯和收缩压均较高。在T2 DM受试者中，GG基因型受试者的空腹血糖显著低于GR + RR基因型受试者。携带IRS-2 Gly 1057 Asp变异的GD + DD基因型的血糖正常受试者的空腹血糖和总胆固醇显著高于携带GG基因型的受试者。我们的研究结果表明，IRS-1 Gly 972 Arg和IRS-2 Gly 1057 Asp多态性与库尔德族T2 DM相关。
An association between the IRS-1 Gly972Arg and IRS-2 Gly1057Asp polymorphisms and type 2 diabetes mellitus (T2DM) in different ethnic groups is controversial. We aimed to identify the association of these polymorphisms with T2DM in the Kurdish ethnic group of Iran. Study groups included 336 T2DM and 341 normoglycemic subjects. Genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism. Genotypic and allelic frequencies were then evaluated. GR and RR genotypes of IRS-1 Gly972Arg variant gave a higher risk for T2DM (odds ratios [OR] = 1.76 and OR = 3.86, respectively). IRS-1 Gly972Arg polymorphism was found to be significantly associated with T2DM (OR = 1.63) for the dominant model (GG vs. GR + RR). GD genotypes of the IRS-2 Gly1057Asp variant gave a higher risk for T2DM (OR = 1.63). The dominant model analysis of the IRS-2 Gly1057Asp genotypes (GG vs. GD + DD) also showed an enhanced association with T2DM (OR = 1.69). Among several combinations, GR/GD gave the highest risk for T2DM (OR = 3.1). Other combinations were also significantly associated with T2DM, including, GR/GG (OR = 1.86), RR/GG (OR = 1.76), GG/GD (OR = 1.83), and GG/DD (OR = 2.35). HbA1c, serum triglyceride, and systolic blood pressure were higher in the control subjects with GR + RR genotypes compared with the GG genotype. Among the T2DM subjects, fasting plasma glucose was significantly lower in subjects with the GG genotype in relation to those with the GR + RR genotypes. Normoglycemic subjects carrying GD + DD genotypes of IRS-2 Gly1057Asp variation had a significantly higher fasting plasma glucose and total cholesterol, as compared with those with the GG genotype. Our findings revealed that IRS-1 Gly972Arg and IRS-2 Gly1057Asp polymorphisms are associated with T2DM in the Kurdish ethnic group.