Triclosan inhibition of fatty acid synthesis and its effect on growth of Escherichia coli and Pseudomonas aeruginosa
Triclosan inhibition of fatty acid synthesis and its effect on growth of Escherichia coli and Pseudomonas aeruginosa
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DOI：
10.1093/jac/dki123
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发表时间：
2005-06-01
影响因子：
5.2
通讯作者：
Ochs, D
中科院分区：
文献类型：
--
作者：
Escalada, MG;Harwood, JL;Ochs, D
目的：评估三氯生对脂肪酸合成的影响，并将烯酰还原酶的抑制与细菌活力联系起来。方法：通过测量总脂质中乙酸盐的掺入量，然后通过气相色谱法分析脂肪酸甲酯，研究三氯生对三氯生耐药大肠杆菌及其敏感对应物和铜绿假单胞菌中脂肪酸合成的影响。同时，评估了三氯生对这些细菌菌株的杀菌效果。结果：三氯生抑制所有测试菌株的脂肪酸生物合成。然而，对于三氯生耐药大肠杆菌（MIC > 1000mg/L），实现抑制所需的浓度高于敏感菌株所需的浓度。这些浓度并未显着影响任何测试菌株的细胞存活。结论：这项研究表明，双酚对脂肪酸生物合成的抑制可能与其生长抑制作用有关，而其他机制也与其致死作用有关。此外，虽然具有高三氯生 MIC 的微生物仍然容易受到双酚对脂肪酸生物合成的抑制作用，但需要更高浓度的化合物。这表明这些菌株中三氯生的生物利用度是不同的。
Objectives: To assess the effect of triclosan on fatty acid synthesis and to relate the inhibition of enoyl reductase to bacterial viability.Methods: The effect of triclosan on fatty acid synthesis in a triclosan-resistant Escherichia coli and its sensitive counterpart and in Pseudomonas aeruginosa was investigated by measuring acetate incorporation into total lipid followed by analysis of fatty acid methyl esters by gas chromatography. Concurrently, the bactericidal effect of triclosan against these bacterial strains was assessed.Results: Triclosan inhibited fatty acid biosynthesis in all the strains tested. However, for triclosan-resistant E. coli (MIC > 1000mg/L) the concentration required to achieve inhibition was higher than that required for the susceptible counterpart. These concentrations did not significantly affect cell survival in any of the strains tested.Conclusions: This study shows that the inhibition of fatty acid biosynthesis by the bisphenol might be involved in its growth-inhibitory action and that other mechanisms are involved in its lethal effect. In addition, although microorganisms with a high triclosan MIC were still susceptible to the inhibitory effect of the bisphenol on fatty acid biosynthesis, a higher concentration of the compound was required. This suggested that triclosan bioavailability was different in these strains.