Comparative study of the protective capacity against Salmonella infection between probiotic and nonprobiotic lactobacilli
Comparative study of the protective capacity against Salmonella infection between probiotic and nonprobiotic lactobacilli
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DOI：
10.1111/jam.12074
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发表时间：
2013-03-01
影响因子：
4
通讯作者：
Perdigon, G.
中科院分区：
文献类型：
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作者：
Castillo, N. A.;de Moreno de LeBlanc, A.;Perdigon, G.
目的在小鼠模型中研究三株乳杆菌对肠道鼠伤寒沙门氏菌的免疫保护能力。确定益生菌的特性，这些特性参与了对该病原体引起的感染的保护。方法和结果3株不同的乳酸杆菌菌株的免疫调节作用：乳酸杆菌（Lactobacillus（Lact.）干酪菌CRL 431（益生菌）、德氏乳杆菌保加利亚亚种CRL 423（保加利亚乳杆菌）和嗜酸乳杆菌CRL 730使用沙门氏菌感染的小鼠模型进行比较。干酪乳杆菌连续给药可提高动物存活率，减少病原体在肠道外的传播，减轻肠道炎症，调节感染前和感染后的细胞因子谱，并增加肠道中伊加的表达和分泌。此外，在体外试验中，这种乳酸杆菌的施用增加了健康小鼠的腹膜、派伊尔集合淋巴结和脾巨噬细胞的吞噬活性以及肠上皮细胞释放的单核细胞趋化蛋白（MCP-1）。尽管嗜酸乳杆菌在感染前和感染后增加了IgA分泌细胞的数量，保加利亚乳杆菌增加了肠上皮细胞释放的MCP-1和巨噬细胞的吞噬活性，但这些作用本身不足以保护小鼠免受鼠伤寒沙门氏菌感染。结论益生菌菌株Lact.caseiCRL431通过增强肠道屏障功能和降低局部炎症反应而诱导对沙门氏菌的保护作用。沙门氏菌研究的意义和影响。构成了世界上食源性疾病的一个重要因素。并非所有的乳酸杆菌，即使在肠道水平具有一些免疫刺激特性，也可以防止沙门氏菌感染。干酪乳杆菌CRL 431是一种益生菌，可用作免疫系统的口腔粘膜佐剂，以改善肠道健康，特别是预防或改善沙门氏菌感染。我们证明，没有一个独特的机制来发挥这种保护作用。
Aims To investigate the immunoprotective ability of three Lactobacilli strains against Salmonella enterica serovar Typhimurium in a mouse model. To identify the probiotic properties involved in the protection against infection caused by this pathogen. Methods and Results The immunomodulatory effect of three different lactobacilli strains: Lactobacillus (Lact.) casei CRL 431 (probiotic bacterium), Lact.delbrueckii subsp.bulgaricus CRL 423 (Lact.bulgaricus) and Lact.acidophilus CRL 730 was compared using a mouse model of Salmonella infection. Lactobacillus casei continuous administration improved animal survival, diminished pathogen spreading outside the intestine, attenuated the intestinal inflammation, modulated cytokine profile previous and postinfection and increased the expression and secretion of IgA in the gut. Additionally, the administration of this lactobacilli increased peritoneal, Peyer's patches and spleen macrophages' phagocytic activity in healthy mice and monocyte chemotactic protein (MCP-1) released by intestinal epithelial cells in an in vitro assay. Although Lact.acidophilus increased the number of IgA-secreting cells previous and postinfection, and Lact.bulgaricus increased MCP-1 released by intestinal epithelial cells and the phagocytic activity of macrophages, these effects alone were not enough to confer protection against Salmonella Typhimurium infection in mouse. Conclusions Probiotic strain Lact.casei CRL 431 was the one that induced protection against Salmonella, by increasing the intestinal barrier function and by decreasing the local inflammatory response. Significance and Impact of the Study Salmonella spp. constitutes an important agent of foodborne diseases in the world. Not all lactobacilli, even with some immunostimulating properties at gut level, can protect against Salmonella infection. Lactobacillus casei CRL 431, a probiotic bacterium, could be useful as an oral mucosal adjuvant of the immune system to improve gut health, especially in the prevention or amelioration of Salmonella infections. We demonstrated that there is not a unique mechanism by which this protective effect was exerted.