Discovery of Point Mutations in the Voltage-Gated Sodium Channel from African Aedes aegypti Populations: Potential Phylogenetic Reasons for Gene Introgression.
Discovery of Point Mutations in the Voltage-Gated Sodium Channel from African Aedes aegypti Populations: Potential Phylogenetic Reasons for Gene Introgression.
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DOI：
10.1371/journal.pntd.0004780
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发表时间：
2016-06
影响因子：
3.8
通讯作者：
Minakawa N
中科院分区：
文献类型：
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作者：
Kawada H;Higa Y;Futami K;Muranami Y;Kawashima E;Osei JH;Sakyi KY;Dadzie S;de Souza DK;Appawu M;Ohta N;Suzuki T;Minakawa N
黄热病在非洲一些国家流行，而伊蚊是与疫情有关的最重要媒介之一。媒介蚊虫全国分布图的绘制和抗药性检测将为登革热和黄热病暴发的预测和有效的控制措施提供有益的信息。在加纳收集的所有蚊子群体中都观察到对滴滴涕的高度抗药性。部分种群怀疑对氯菊酯产生了抗性，并可能存在抗性或耐受性。检测到电压门控钠通道（F1534C）和另一突变（V1016I）的一个杂合子的高频率点突变，这是非洲大陆的首次检测。F1534C等位基因频率和纯合子比例在Ae.埃及伊蚊（Aaa. aegypti aegypti，Aaa.）埃及伊蚊（Aaf.）在第20和21外显子之间检测到两种类型的内含子，其中F1534C突变与一种常见于东南亚和中南美洲国家的内含子紧密连锁，提示该突变可能是从其他大陆引入的，或者是在上述地区Aaa基因渗入后发生的聚合选择。20世纪40年代和50年代的全球性消灭计划可能对蚊子种群造成了很高的选择压力，并扩大了抗药性Ae的分布。埃及人。可以假设F1534C点突变的选择在此期间发生。抗源的选择。埃及伊蚊携带F1534C点突变，以及随着人类活动如旧轮胎贸易的进行，媒介蚊虫在世界范围内的传播，可能导致F1534C点突变在热带国家广泛分布。埃及伊蚊是黄热病和登革热最重要的传播媒介之一。拟除虫菊酯类杀虫剂正在成为病媒控制的主要杀虫剂，病媒蚊虫对拟除虫菊酯类杀虫剂的抗药性是病媒控制计划的主要问题。据报道，电压门控钠通道的几个突变在埃及伊蚊对拟除虫菊酯的抗性中起重要作用。最近，一种新的F1534C突变被报道与DDT和拟除虫菊酯抗性密切相关。我们观察到高抗DDT和中度抗氯菊酯在这两个Ae。埃及伊蚊Aegypti aegypti（Aaa）和埃及伊蚊Ae.在加纳收集的埃及丽纹伊蚊（Aaf）菌落。同时，在上述蚊子群体中发现了高频率的F1534C突变，这是非洲大陆首次发现。我们发现F1534C突变和外显子20和21之间的内含子之间存在强烈的连锁关系，常见于东南亚和南美洲和中美洲国家。加纳山羊对DDT和拟除虫菊酯的抗性。埃及伊蚊种群中的Aaa基因可能是来自上述地区的Aaa基因渗入所致。
Yellow fever is endemic in some countries in Africa, and Aedes aegpyti is one of the most important vectors implicated in the outbreak. The mapping of the nation-wide distribution and the detection of insecticide resistance of vector mosquitoes will provide the beneficial information for forecasting of dengue and yellow fever outbreaks and effective control measures. High resistance to DDT was observed in all mosquito colonies collected in Ghana. The resistance and the possible existence of resistance or tolerance to permethrin were suspected in some colonies. High frequencies of point mutations at the voltage-gated sodium channel (F1534C) and one heterozygote of the other mutation (V1016I) were detected, and this is the first detection on the African continent. The frequency of F1534C allele and the ratio of F1534C homozygotes in Ae. aegypti aegypti (Aaa) were significantly higher than those in Ae. aegypti formosus (Aaf). We could detect the two types of introns between exon 20 and 21, and the F1534C mutations were strongly linked with one type of intron, which was commonly found in South East Asian and South and Central American countries, suggesting the possibility that this mutation was introduced from other continents or convergently selected after the introgression of Aaa genes from the above area. The worldwide eradication programs in 1940s and 1950s might have caused high selection pressure on the mosquito populations and expanded the distribution of insecticide-resistant Ae. aegypti populations. Selection of the F1534C point mutation could be hypothesized to have taken place during this period. The selection of the resistant population of Ae. aegypti with the point mutation of F1534C, and the worldwide transportation of vector mosquitoes in accordance with human activity such as trading of used tires, might result in the widespread distribution of F1534C point mutation in tropical countries. Aedes aegpyti is one of the most important vectors of yellow fever and dengue fever. Pyrethroid insecticides are emerging as the predominant insecticides for vector control, and resistance of vector mosquitoes to pyrethroid is a major problem for the vector control program. Several mutations in the voltage-gated sodium channel were reported to play important roles in pyrethroid resistance of Aedes aegypti. Recently, a novel F1534C mutation was reported to be strongly correlated with resistance to DDT and pyrethroid. We observed a high resistance to DDT and moderate resistance to permethrin in both Ae. aegypti aegypti (Aaa) and Ae. aegypti formosus (Aaf) colonies collected in Ghana. Concurrently, high frequencies of F1534C mutations were found in the above mosquito colonies, and this was its first detection on the African continent. We found a strong linkage of F1534C mutation and the introns between exon 20 and 21 commonly found in South East Asian and South and Central American countries. The DDT and pyrethroid resistance in Ghanaian Ae. aegypti population was suggested to be caused by the introgression of Aaa genes from the above area.