Development of Anncaliia algerae (Microsporidia) in Drosophila melanogaster
Development of Anncaliia algerae (Microsporidia) in Drosophila melanogaster
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DOI：
10.1111/jeu.12762
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发表时间：
2019-10-13
影响因子：
2.2
通讯作者：
DiMario, Patrick J.
中科院分区：
文献类型：
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作者：
Sokolova, Yuliya Y.;Weidner, Earl;DiMario, Patrick J.
Anncaliia属的代表被称为双翅目和鞘翅目昆虫，片足类甲壳动物的天然寄生虫，但也是人类，主要是免疫缺陷。由藻类Anncalia algerae引起的致命性肌炎被认为是人类的一种紧急传染病。A.（=Nosema，Brachiola）algerae是微孢子虫属中研究最多的一种，在自然界和实验中的微孢子虫宿主范围最广，但从未在果蝇中繁殖过。本文对A. algerae在果蝇内脏肌肉和脂肪细胞中的作用。我们观察到典型的Anncaliia spp。超微结构和细胞病理学特征，包括孢子形态、质膜的特征性延伸以及在增殖阶段存在“脊”和管状物质的附属物。Anncaliia algerae的发育过程中，D. melanogaster与A. algerae和A.（Brachiola）vesicularum在人类急性肌炎中的作用给定D.黑腹果蝇是目前最成熟的遗传模型，其基因组全序列、易获得的转基因形式和基因组标记，新的宿主-寄生虫系统可能为研究黑腹果蝇的宿主-病原相互作用提供新的遗传工具。阿尔及利亚，以及测试抗微孢子虫药物。
Representatives of the genus Anncaliia are known as natural parasites of dipteran and coleopteran insects, amphipod crustaceans, but also humans, primarily with immunodeficiency. Anncaliia algerae-caused fatal myositis is considered as an emergent infectious disease in humans. A. (=Nosema, Brachiola) algerae, the best studied species of the genus, demonstrates the broadest among microsporidia range of natural and experimental hosts, but it has never been propagated in Drosophila. We present ultrastructural analysis of development of A. algerae in visceral muscles and adipocytes of Drosophila melanogaster 2 wk after per oral experimental infection. We observed typical to Anncaliia spp. features of ultrastructure and cell pathology including spore morphology, characteristic extensions of the plasma membrane, and presence of "ridges" and appendages of tubular material at proliferative stages. Anncaliia algerae development in D. melanogaster was particularly similar to one of A. algerae and A.(Brachiola) vesicularum in humans with acute myositis. Given D. melanogaster is currently the most established genetic model, with a fully sequenced genome and easily available transgenic forms and genomic markers, a novel host-parasite system might provide new genetic tools to investigate host-pathogen interactions of A. algerae, as well to test antimicrosporidia drugs.