Analysis of HOX Gene Expression Patterns in Human Breast Cancer
Analysis of HOX Gene Expression Patterns in Human Breast Cancer
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DOI：
10.1007/s12033-013-9682-4
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发表时间：
2014-01-01
影响因子：
2.6
通讯作者：
Kim, Myoung Hee
中科院分区：
文献类型：
--
作者：
Hur, Ho;Lee, Ji-Yeon;Kim, Myoung Hee
HOX基因是高度保守的转录因子，其决定发育中胚胎中沿前-后体轴沿着的细胞和组织的身份。HOX基因表达的异常已在多种肿瘤中显示。然而，HOX基因表达模式与肿瘤发生和癌症进展的相关性尚未得到充分表征。在这里，为了分析参与乳腺癌肿瘤发生和发展的推定候选HOX基因，使用乳腺癌细胞系和患者来源的乳腺组织分析了39个HOX基因的表达模式。体外分析显示，HOXA和HOXB基因表达发生在一个亚型特异性的方式在乳腺癌细胞系，而大多数HOXC基因强烈表达在大多数细胞系。在分析的39个HOX基因中，选择25个用于在恶性和非恶性组织中的进一步分析。在25个基因中，编码HOXA 6、A13、B2、B4、B5、B6、B7、B8、B 9、C5、C9、C13、D1和D8的14个基因在非恶性和恶性乳腺组织之间显示出统计学显著的差异表达模式，并且是与乳腺癌的发展和恶性进展相关的推定候选基因。我们的数据为进一步了解HOX基因在乳腺癌中的表达以及HOX基因可能参与肿瘤进展提供了宝贵的资源。
HOX genes are highly conserved transcription factors that determine the identity of cells and tissues along the anterior-posterior body axis in developing embryos. Aberrations in HOX gene expression have been shown in various tumors. However, the correlation of HOX gene expression patterns with tumorigenesis and cancer progression has not been fully characterized. Here, to analyze putative candidate HOX genes involved in breast cancer tumorigenesis and progression, the expression patterns of 39 HOX genes were analyzed using breast cancer cell lines and patient-derived breast tissues. In vitro analysis revealed that HOXA and HOXB gene expression occurred in a subtype-specific manner in breast cancer cell lines, whereas most HOXC genes were strongly expressed in most cell lines. Among the 39 HOX genes analyzed, 25 were chosen for further analysis in malignant and non-malignant tissues. Fourteen genes, encoding HOXA6, A13, B2, B4, B5, B6, B7, B8, B9, C5, C9, C13, D1, and D8, out of 25 showed statistically significant differential expression patterns between non-malignant and malignant breast tissues and are putative candidates associated with the development and malignant progression of breast cancer. Our data provide a valuable resource for furthering our understanding of HOX gene expression in breast cancer and the possible involvement of HOX genes in tumor progression.