ISOLATION OF THE CHICKEN THYMIDINE KINASE GENE BY PLASMID RESCUE
ISOLATION OF THE CHICKEN THYMIDINE KINASE GENE BY PLASMID RESCUE
复制标题
DOI：
10.1038/285207a0
复制
发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
WIGLER, M
中科院分区：
文献类型：
--
作者：
PERUCHO, M;HANAHAN, D;WIGLER, M
细菌质粒pBR 322用作载体以从高等真核生物分离编码选择标记的基因。鸡胸苷激酶（tk）基因作为2.2-磷酸酶EcoRI/HindIII插入物在pBR 322中获得。克隆的基因转化tk-动物细胞的效率与克隆的单纯疱疹病毒-1 tk基因的效率相等。
The bacterial plasmid pBR322 was used as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. The chicken thymidine kinase (tk) gene was obtained as a 2.2-kilobase EcoRI/HindIII insert in pBR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.