Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization
Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization
复制标题
DOI：
10.1016/j.molcel.2021.02.006
复制
发表时间：
2021-03-18
期刊：
影响因子：
16
通讯作者：
Spahn, Christian M. T.
中科院分区：
文献类型：
--
作者：
Nikolay, Rainer;Hilal, Tarek;Spahn, Christian M. T.
核糖体生物发生是一个基本的多步骤细胞过程，最终形成核糖体亚基，其产生和修饰受许多生物发生因素的调节。在这项研究中，我们从大肠杆菌菌株中分离出真实的pre-50S亚基，并在其天然基因位点上标记了生物发生因子ObgE，从而分析了生理性核糖体的生物发生。我们的综合结构方法揭示了一个由YjgA、RluD、RsfS和ObgE组成的相互作用的生物发生因子网络，这些因子位于未成熟的pre-50S亚基上。此外，我们的研究为GTPase ObgE如何与其他生物发生因子协同促进50S功能核心的成熟提供了机制见解，并揭示了原核生物和真核生物之间核糖体生物发生的保守和不同的进化特征。
Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.