Numeric aberrations of HER-2 and chromosome 17 detected by fluorescence in situ hybridization in urine-exfoliated cells from patients with urothelial carcinoma
Numeric aberrations of HER-2 and chromosome 17 detected by fluorescence in situ hybridization in urine-exfoliated cells from patients with urothelial carcinoma
复制标题
DOI：
10.1016/j.urology.2004.05.004
复制
发表时间：
2004-09-01
期刊：
影响因子：
2.1
通讯作者：
Kato, T
中科院分区：
文献类型：
--
作者：
Inoue, T;Sato, K;Kato, T
目标。目的 阐明荧光原位杂交（FISH）检测尿路上皮移行细胞癌患者尿液脱落细胞中 HER-2 基因改变的临床意义。方法。使用 103 名患者的 HER-2 和 17 号染色体着丝粒 DNA 探针检查尿液脱落细胞中 HER-2 (RI-HER2) 的相对增加和 17 号染色体 (G-17) 的增加。此外，使用45例相应的石蜡包埋组织切片进行FISH分析，以比较使用尿液脱落细胞和使用石蜡包埋组织获得的结果。 103 名患者中分别有 23 名 (22.3%) 和 46 名 (44.6%) 发现 RI-HER2 和 G-I 7。 RI-HER2 在两次或两次以上复发的肿瘤中显着更高（40.7% 对比 15.8%，P = 0.010）和原位癌 (CIS) 肿瘤（35.4% 对比 15.9%，P = 0.029）。 G-17 在高级别肿瘤（69.1% vs 16.7%，P = 0.032）、侵袭性肿瘤（63.6% vs 14.3%，P < 0.001）和 CIS 患者（77.1% vs 29.0%，P < 0.001）中更为常见。 FISH 的阳性率（存在 RI-HER2 和/或存在 G-I 7）在 FISH 中比在细胞学中更常见。使用尿液脱落细胞和石蜡包埋组织进行的分析比较显示，45 例中有 36 例（80.0%）结果相同。结论。 FISH 检测到的尿液脱落细胞中 17 号染色体着丝粒的数字变化可能反映了尿路上皮癌的恶性潜力。此外，HER-2 的相对增加与复发次数和 CIS 的存在相关。
Objectives. To elucidate the clinical significance of the HER-2 gene alterations in urine-exfoliated cells detected by fluorescence in situ hybridization (FISH) in patients with urothelial transitional cell carcinoma.Methods. The relative increase of HER-2 (RI-HER2) and gain of chromosome 17 (G-17) in urine-exfoliated cells were examined using DNA probes for HER-2 and the chromosome 17 centromere in 103 patients. In addition, FISH analysis was performed using corresponding paraffin-embedded tissue sections from 45 cases to compare the results obtained using urine-exfoliated cells and those obtained using paraffin-embedded tissue.Results. RI-HER2 and G-I 7 was found in 23 (22.3%) and 46 (44.6%) of 103 patients, respectively. RI-HER2 was significantly more frequent in tumors with two or more recurrences (40.7% versus 15.8%, P = 0.010) and in those with carcinoma in situ (CIS) (35.4% versus 15.9%, P = 0.029). G-17 was more frequent in high-grade tumors (69.1% versus 16.7%, P = 0.032), invasive tumors (63.6% versus 14.3%, P < 0.001), and in patients with CIS (77.1% versus 29.0%, P < 0.001). The positive rate for FISH (presence of RI-HER2 and/or presence of G-I 7) tended to be more frequent in FISH than in cytology. A comparison of the analyses using urine-exfoliated cells and paraffin-em bedded tissue showed identical results in 36 (80.0%) of 45 cases.Conclusions. Numeric alterations of the chromosome 17 centromere in urine-exfoliated cells detected by FISH may reflect the malignant potential of urothelial carcinoma. In addition, a relative increase in HER-2 was associated with the number of recurrences and the presence of CIS.