Electron microscopy of G-banded human mitotic chromosomes.
Electron microscopy of G-banded human mitotic chromosomes.
复制标题
G 带人类有丝分裂染色体的电子显微镜。
作者：
Xu,X;Wu,M
经胰蛋白酶处理的人中期染色体经吉氏染色和醋酸铀酰染色，透射电镜下显示清晰、可复制的条带结构。透射电镜观察到的条带模式与光学显微镜观察到的g带模式非常接近。透射电镜图像用于核型分析。用醋酸铀酰单独染色胰蛋白酶处理的染色体在透射电镜下也显示出清晰的g带。除了醋酸铀酰染色外，阴影投射还揭示了染色体的更多结构细节。在带间区观察到直径200 Å-300 Å的染色体纤维。在没有胰蛋白酶处理的情况下，大多数染色体在高倍电镜下显示主要的g带。
Trypsin-treated human metaphase chromosomes stained with Giemsa and uranyl acetate showed clear, reproducible band structures under the transmission electron microscope (TEM). The banding pattern observed with TEM corresponded very closely to the G-band pattern visualized by light microscopy. The TEM images were used for karyotype analyses. Trypsin-treated chromosomes stained with uranyl acetate alone also showed clear G-bands under TEM. Shadow casting in addition to uranyl acetate staining revealed more structural detail of the chromosomes. Chromosome fibers, 200 Å–300 Å in diameter, were observed in the interband regions. Most chromosomes showed the major G-bands under the higher TEM magnification wit0out any trypsin treatment.