THE FIBRINOGEN MOLECULE - ITS SIZE, SHAPE, AND MODE OF POLYMERIZATION
THE FIBRINOGEN MOLECULE - ITS SIZE, SHAPE, AND MODE OF POLYMERIZATION
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DOI：
10.1083/jcb.5.1.11
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发表时间：
1959-01-01
期刊：
影响因子：
--
通讯作者：
SLAYTER, HS
中科院分区：
文献类型：
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作者：
HALL, CE;SLAYTER, HS
改进的电子显微照片的阴影铸造牛纤维蛋白原分子已获得建立其一般形态和尺寸在干燥状态。它由3个结节的线性阵列组成，由一根非常细的线连接在一起，估计直径为8至15 A，但尚未明确分辨。两个末端结节相似，但中心结节略小。阴影长度的测量表明结节直径在50至70埃的范围内。干燥分子的长度是475 [正负] 25埃。采用以往理化数据的分子体积和电镜观察的一般形态特征和长度，计算出末端结节的直径为65，中心结节的直径为50。所得分子模型与电子显微镜观察和物理化学数据一致。还检查了当纤维蛋白原用凝血酶活化时形成的中间聚合物，发现其是改变的纤维蛋白原分子的端对端聚集体，其在该过程中长度收缩。中间聚合物长度为1000至5000 A。纤维蛋白原的结节性质，它的收缩和端到端的聚合聚合使我们能够推断出一个解释系统的交叉带以前观察到的染色纤维蛋白原纤维。
Improved electron micrographs of the shadow-cast bovine fibrinogen molecule have been obtained establishing its general morphology and dimensions in the dry state. It consists of a linear array of 3 nodules held together by a very thin thread which is estimated to have a diameter of from 8 to 15 A, though it is not clearly resolved. The two end nodules are alike but the center one is slightly smaller. Measurements of shadow lengths indicate that nodule diameters are in the range 50 to 70 A. The length of the dried molecule is 475 [plus or minus] 25 A. Adopting the molecular volume from previous physical chemical data and the general morphological features and length from electron microscopy, we calculate the diameters of the end nodules to be 65 A and the center one as 50 A. The model of the molecule so obtained is consistent with the electron microscopical observations and the data from physical chemistry. The intermediate polymers formed when fibrinogen is activated with thrombin were also examined and found to be end-to-end aggregates of altered fibrinogen molecules which shrink in length during the process. Intermediate polymer lengths are from 1000 to 5000 A. The nodular nature of fibrinogen, its shrinkage and end-to-end aggregation on polymerization permits us to deduce an explanation for the system of cross-bands previously observed in stained fibrin fibrils.