A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization
A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization
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DOI：
10.1364/boe.1.000186
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发表时间：
2010-08-02
影响因子：
3.4
通讯作者：
Applegate, Brian E.
中科院分区：
文献类型：
--
作者：
Park, Jesung;Jo, Javier A.;Applegate, Brian E.
大多数病理状况引起组织光学响应的变化，其可以通过一种或多种光学成像模态来询问。任何单一的模态通常只会显示组织光学响应的不完整图像，因此需要一种集成互补光学成像模态的方法来进行更全面的非破坏性和微创组织表征。我们已经开发出一种双模态系统，将光学相干断层扫描（OCT）和荧光寿命成像显微镜（FLIM），这是能够同时表征的3-D组织形态和其生化成分。傅立叶域OCT子系统，在830 nm的中心波长，提供了高分辨率的形态体积组织图像的轴向和横向分辨率分别为7.3和13.4 μ m。多光谱FLIM子系统基于直接脉冲记录方法（在355 nm激光激发下），提供了三个可定制发射带处组织自发荧光强度和寿命的二维表面图，横向分辨率为100 μ m。两个子系统共享相同的激发/照明光路，并在样本上同时进行光栅扫描，以生成共配准的OCT体积和FLIM图像。所开发的OCT/FLIM系统能够实现OCT的最大A线速率为59 KHz，FLIM的像素速率高达30 KHz。双模态系统进行了验证与标准的荧光团解决方案，并随后适用于两种生物组织类型的表征：死后的人类冠状动脉粥样硬化斑块，并在体内正常和癌变仓鼠颊囊上皮组织。(C)2010年美国光学学会
Most pathological conditions elicit changes in the tissue optical response that may be interrogated by one or more optical imaging modalities. Any single modality typically only furnishes an incomplete picture of the tissue optical response, hence an approach that integrates complementary optical imaging modalities is needed for a more comprehensive non-destructive and minimally-invasive tissue characterization. We have developed a dual-modality system, incorporating optical coherence tomography (OCT) and fluorescence lifetime imaging microscopy (FLIM), that is capable of simultaneously characterizing the 3-D tissue morphology and its biochemical composition. The Fourier domain OCT subsystem, at an 830 nm center wavelength, provided high-resolution morphological volumetric tissue images with an axial and lateral resolution of 7.3 and 13.4 mu m, respectively. The multispectral FLIM subsystem, based on a direct pulse-recording approach (upon 355 nm laser excitation), provided two-dimensional superficial maps of the tissue autofluorescence intensity and lifetime at three customizable emission bands with 100 mu m lateral resolution. Both subsystems share the same excitation/illumination optical path and are simultaneously raster scanned on the sample to generate coregistered OCT volumes and FLIM images. The developed OCT/FLIM system was capable of a maximum A-line rate of 59 KHz for OCT and a pixel rate of up to 30 KHz for FLIM. The dual-modality system was validated with standard fluorophore solutions and subsequently applied to the characterization of two biological tissue types: postmortem human coronary atherosclerotic plaques, and in vivo normal and cancerous hamster cheek pouch epithelial tissue. (C) 2010 Optical Society of America