Functional annotation of expressed sequence tags as a tool to understand the molecular mechanism controlling flower bud development in tree peony.
Functional annotation of expressed sequence tags as a tool to understand the molecular mechanism controlling flower bud development in tree peony.
复制标题
DOI：
10.1111/j.1399-3054.2009.01206.x
复制
发表时间：
2009-04
影响因子：
6.4
通讯作者：
Q. Shu;Elisabeth Wischnitzki;Z. Liu;H. Ren;Xiao-yan Han;Qing Hao;Fenghua Gao;Suying Xu;Liang‐Sheng Wang
中科院分区：
文献类型：
--
作者：
Q. Shu;Elisabeth Wischnitzki;Z. Liu;H. Ren;Xiao-yan Han;Qing Hao;Fenghua Gao;Suying Xu;Liang‐Sheng Wang
牡丹是一种重要的药用和观赏植物。在中国，它的根皮是传统中药的重要成分。它作为一种观赏植物的价值，因为它的花在形状和颜色上表现出广泛的变化。我们利用不同发育阶段的花蕾构建了该生物的第一个cDNA文库。单向测序后，共获得2241个原始表达序列标签（EST）。经过处理和组装，他们总共产生了1300个单基因[363个重叠群，平均大小为3.5个EST（范围从2到36）和937个单基因]。根据相似的功能特征或细胞作用，将基因本体分类分配并进一步总结为13个具有生物学作用的大家族。所有重叠群共检测到185个单核苷酸多态性。我们能够检测到开放阅读框架的共识序列的1268 unigenes。97.5%的EST序列与公共数据库中的序列具有显著的相似性。在整个数据集中获得了167个短序列重复。这些分析和信息将为更好地了解这一重要植物提供有价值的资源，也可用于该科植物的功能研究。
Tree peony (Paeonia suffruticosaAndrews) is an important medicinal and ornamental plant. In China, its root bark is an important ingredient for traditional Chinese medicine. It is valued as an ornamental plant because its flower shows a wide variation in shape and color. We used flower buds at different developmental stages to construct the first cDNA library for this organism. A total of 2241 raw expressed sequence tags (ESTs) were obtained after unidirectional sequencing. After processing and assembly, they resulted in a total of 1300 unigenes [363 contigs with an average size of 3.5 ESTs (ranging from 2 up to 36) and 937 singletons]. Gene Ontology categories were assigned and further summarized into 13 broad families with biological roles according to similar functional characteristics or cellular roles. A total of 185 single nucleotide polymorphisms were detected for all contigs. We were able to detect open reading frames in the consensus sequences of 1268 unigenes. 97.5% of the ESTs showed significant similarity to sequences present in public databases. One hundred and sixty-seven short sequence repeats were obtained in the whole data set. All the analysis and information will be valuable resources for a better understanding of this important plant and also can be used for functional study in Paeoniaceae.