Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials
Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials
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DOI：
10.1021/bp020071x
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发表时间：
2002-11-01
影响因子：
2.9
通讯作者：
Banat, IM
中科院分区：
文献类型：
--
作者：
Rahman, KSM;Rahman, TJ;Banat, IM
本研究的目的是通过建议使用低成本的原材料来开发经济的方法以获得更高产率的生物表面活性剂。两个石油降解菌株，铜绿假单胞菌QS 9 -119和DS 10 -129，用于优化底物的最大鼠李糖脂生产。在这两种菌株中，后者产生的最大值为4.31，2.98和，1.77 g/L的鼠李糖脂生物表面活性剂使用大豆。油、红花油和甘油。生物表面活性剂的产率。甚至在细菌培养物达到生长的稳定期后也稳定增加。使用质谱法表征鼠李糖脂，发现存在二鼠李糖脂（Rha-Rha-C-10-C-10）。铜绿假单胞菌DS 10 -129产生的鼠李糖脂生物表面活性剂的乳化活性使用所有测试的烃（包括二甲苯、苯、己烷、原油、煤油、汽油和柴油）大于70%。GS 9 -119仅将己烷和煤油乳化至该水平。
This study was aimed at the development of economical methods for higher yields of biosurfactant by suggesting the use of low-cost raw materials. Two oil-degrading strains, Pseudomonas aeruginosa QS9-119 and DS10-129, were used to optimize a substrate for maximum rhamnolipid production. Among the two strains, the latter produced maxima of 4.31, 2.98, and, 1.77 g/L rhamnolipid biosurfactant using soybean. oil, safflower oil, and glycerol, respectively. The yield of biosurfactant. steadily increased even after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids (Rha-Rha-C-10-C-10). Emulsification activity of the rhamnolipid biosurfactant produced by P. aeruginosa DS10-129 was greater than 70% using all the hydrocarbons tested, including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P. aeruginosa.GS9-119 emulsified only hexane and kerosene to that level.