The nectary-specific pattern of expression of the tobacco Nectarin I promoter is regulated by multiple promoter elements
The nectary-specific pattern of expression of the tobacco Nectarin I promoter is regulated by multiple promoter elements
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DOI：
10.1023/a:1022370203570
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发表时间：
2003-03-01
影响因子：
5.1
通讯作者：
Thornburg, RW
中科院分区：
文献类型：
--
作者：
Carter, C;Thornburg, RW
烟草植物花蜜中分泌的主要蛋白质（Nectarin I）是一种具有超氧化物歧化酶活性的萌发样蛋白质。我们分离了编码油桃素I（NECI）的基因，并分析了由其启动子驱动的氯霉素乙酰转移酶（CAT）标记基因在转基因植物中的表达。在全长NECI启动子控制下表达CAT基因的转基因植物株系在成熟花蜜腺中表现出高水平的CAT表达。NECI-CAT表达的组织特异性表明，该构建体在蜜腺中唯一表达，在卵巢中表达水平很低。此外，其时间表达的分析表明，该构建体在花蜜从花中积极分泌的那些时间期间独特地表达。对转录起始位点的检测证实，转基因植物中NECI-CAT mRNA的起始位点与体内天然基因的起始位点相同。还制备并分析了两个启动子缺失构建体。对转基因植株的分析表明，花蜜特异性表达是多个启动子元件共同调控的结果，表明花蜜分泌和花开放可能是协同调控的。
The major protein secreted into the nectar of tobacco plants (Nectarin I) is a germin-like protein that has superoxide dismutase activity. We have isolated the gene encoding Nectarin I (NECI) and analyzed the expression of a chloramphenicol acetyl transferase (CAT) marker gene driven by its promoter in transgenic plants. Transgenic plant lines that expressed the CAT gene under control of the full-length NECI promoter showed high levels of CAT expression in mature floral nectaries. Tissue specificity of NECI-CAT expression demonstrated that the construct was expressed uniquely in nectaries with a small level of expression in ovary. Further, analysis of its temporal expression showed that the construct is expressed uniquely during those times when nectar is actively being secreted from flowers. An examination of the transcription start site verified that the initiation site of the NECI-CAT mRNA in transgenic plants is identical with that of the native gene in vivo. Two promoter deletion constructs were also prepared and analyzed. Analysis in transgenic plants revealed that the nectary-specific expression is the result of multiple promoter elements and suggests that nectar secretion and flower opening may be coordinately regulated.