Characterization of molluscan phagocyte subpopulations based on lysosomal enzyme markers
Characterization of molluscan phagocyte subpopulations based on lysosomal enzyme markers
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DOI：
10.1002/jez.1402260205
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发表时间：
1983-05
影响因子：
--
通讯作者：
W. O. Granath;T. Yoshino
中科院分区：
文献类型：
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作者：
W. O. Granath;T. Yoshino
本研究定量分析了两种钉螺（Biomphalaria）吞噬细胞（血细胞）内及之间溶酶体酶标记物、酸性磷酸酶（AP）、非特异性酯酶（NE）和过氧化物酶（PO）的分布和丰度。人类血吸虫，曼氏血吸虫的媒介宿主。在血吸虫耐药（10-R2）和易感（PR白化）的蜗牛株的玻璃贴壁血细胞群中发现了AP-、NE-和po -染色细胞的离散亚群。此外，在蜗牛种群内部和不同菌株之间，定义不同亚群的酶的分布和丰度也存在显著差异。通常，10-R2血细胞比PR白化细胞具有更高的酶活性，这在一定程度上可归因于10-R2菌株中较大的NE-only、AP-only和AP + ne染色的血细胞，以及PR白化蜗牛中较大的阴性细胞亚群。此外，与PR白化蜗牛相比，10-R2菌株具有几乎两倍的循环玻璃粘附血细胞，这一事实进一步增强了血细胞酶含量的差异。这些发现可能具有额外的意义，因为先前的研究表明血细胞溶酶体酶与杀死天然抗性10-R2蜗牛中的血吸虫幼虫有关，尽管尚未对这些细胞中的菌株特异性酶水平进行精确测量。本研究提供了直接证据，表明10-R2蜗牛天然具有比PR白化菌株更高的溶酶体酶含量，这反过来可能与这两种软体动物菌株所观察到的抗性或易感性有关。
This study quantitatively analyzed the distribution and abundance of the lysosomal enzyme markers, acid phosphatase (AP), nonspecific esterase (NE), and peroxidase (PO) within and between the phagocytes (hemocytes) of two strains of the snail, Biomphalaria. Glabrata, vector host for the human blood fluke, Schistosoma mansoni. Discrete subpopulations of AP-, NE-, and PO-staining cells were found in the glass-adherent hemocyte population of both schistosome-resistant (10-R2) and susceptible (PR albino) strains of the snail. Furthermore, there were significant differences in the distribution and abundance of the different enzymes defining the subpopulations within and between the snail strains. Generally, 10-R2 hemocytes have a higher enzymatic activity than PR albino cells, which, in part, can be attributed to larger NE-only, AP-only, and AP + NE-staining hemocytes in the 10-R2 strain, and a larger subpopulation of negative cells in PR albino snails. Moreover, these differences in hemocyte enzyme content are further augmented by the fact that the 10-R2 strain has nearly twice as many circulating, glass-adherent blood cells when compared to PR albino snails. These findings may be of additional significance, since previous studies have implicated hemocyte lysosomal enzymes in the killing of schistosome larvae in naturally resistant 10-R2 snails, although accurate measurements of strain-specific enzyme levels in these cells have not been conducted. The present study provides direct evidence that 10-R2 snails naturally possess a much greater lysosomal enzyme content than do those of the PR albino strain, which, in turn, may be related to the observed resistance or susceptibility in these two molluscan strains.