Engineered co-culture for consolidated production of phenylpropanoids directly from aromatic-rich biomass.
Engineered co-culture for consolidated production of phenylpropanoids directly from aromatic-rich biomass.
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DOI：
10.1016/j.biortech.2023.129935
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发表时间：
2023-11
影响因子：
11.4
通讯作者：
Micaela Chacόn;Ellen Percival;T. Bugg;N. Dixon
中科院分区：
文献类型：
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作者：
Micaela Chacόn;Ellen Percival;T. Bugg;N. Dixon
将生物质原料就地水解和转化为增值产品的综合生物工艺为降低工艺和成本提供了巨大的潜力。然而，迄今为止，已经开发了几种针对富含芳族化合物的原料馏分的综合生物工艺。本文报道了玉米芯羟基肉桂酸的合成共培养的发展。生物质水解是通过在B中开发的分泌模块实现的。subtilisusing使用a genetically基因encoded编码biosensor-actuator生物传感器to secrete分泌hydrolytic水解enzymes酶.通过E开发的生物转化模块实现转化。大肠杆菌使用一套即插即用的编码酶将释放的羟基肉桂酸转化为高价值的苯丙素类目标化合物。最后，通过纤维素酶预处理、萃取发酵和产物回收，从同一工艺中分离得到高纯度的松柏醇和胡椒酚。
Consolidated bioprocesses for thein situhydrolysis and conversion of biomass feedstocks into value-added products offers great potential for both process and cost reduction. However, to date few consolidated bioprocesses have been developed that target aromatic rich feedstock fractions. Reported here is the development of synthetic co-cultivation for the consolidated hydrolysis and valorisation of corncob hydroxycinnamic acids. Biomass hydrolysis was achieved via a secretion module developed inB. subtilisusing a genetically encoded biosensor-actuator to secrete hydrolytic enzymes. Conversion was achieved via a biotransformation module developed inE. coliusing a suite of plug-and-play encoded enzymes to convert the released hydroxycinnamic acids into high-value phenylpropanoid target compounds. Finally, employing cellulolytic pre-treatment, extractive fermentation andin situproduct recovery multiple aromatic products, coniferol and chavicol, were isolated from the same process in high purity.