Molecular detection of divergent trypanosomes among rodents of Thailand
Molecular detection of divergent trypanosomes among rodents of Thailand
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DOI：
10.1016/j.meegid.2007.08.002
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发表时间：
2008-07-01
影响因子：
3.2
通讯作者：
Stich, R. W.
中科院分区：
文献类型：
--
作者：
Jittapalapong, S.;Inpankaew, T.;Stich, R. W.
Herpetosoma是一个同质的亚属，有几十个命名的物种，通常被描述为形态上难以区分的T。类似刘易斯的寄生虫。这些锥虫通常感染啮齿动物并利用跳蚤作为载体。虽然这种锥虫亚属被认为是非致病性的正常主机，其中一些是在罕见的情况下报告与人类疾病。最近，一个T。在一名泰国婴儿中检测到lewisi样感染，因此本研究的目的是调查T。在泰国本土的不同啮齿类动物中进行lewisi感染的研究，以确定人类病例的可能来源。2006年至2007年期间，从泰国三个省的城市和农村地区共收集了276只啮齿动物的血液。对这些样本进行DNA分离处理，并使用锥虫属通用的PCR检测方法进行检测，然后进行内部转录间隔区1（ITS-1）序列分析，以鉴定阳性样本中的感染。被称为T.鼠（14.3%）和板齿动物（18.0%）中存在Lewisi样锥虫;伊氏疟原虫在Leopoldamys（20%）和Rattus（2.0%）种中检出。疱疹流行的啮齿动物与人类和森林栖息地，而三个唾液阳性的啮齿动物来自森林群落。从其中一个样品中扩增的疱疹病毒ITS-1序列与报道的T. lewisi在实验感染的大鼠中扩增的序列与从泰国婴儿的血液中扩增的序列具有96.4%的相同性。采集啮齿动物的场所对啮齿动物感染有显著影响，至少对T。lewisi，提示人类化程度可能影响锥虫的传播。这些结果表明，多个Herpetosoma物种或菌株是地方流行的泰国，和鼠和板齿门物种是人类接触这些寄生虫的可能来源。(C)2007年由Elsevier B. V.出版。
Herpetosoma is a homogenous subgenus of several dozen named species that are often described as morphologically indistinguishable T. lewisi-like parasites. These trypanosomes normally infect rodents and utilize fleas as vectors. Although this trypanosome subgenus is considered nonpathogenic to normal hosts, some of them are on rare occasion reported in association with human disease. Recently, a T. lewisi-like infection was detected in a sick Thai infant, thus the objective of this study was to investigate the prevalence of T. lewisi infections among different rodents indigenous to Thailand in order to identify possible sources of human cases. Blood was collected from a total of 276 rodents trapped from urban and rural areas of three Thai provinces between 2006 and 2007. These samples were processed for DNA isolation and tested with a PCR assay universal for the genus Trypanosoma, followed by internal transcribed spacer 1 (ITS-1) sequence analysis to identify infections in positive samples. Herpetosoma known as T. lewisi-like trypanosomes were present among Rattus (14.3%) and Bandicota (18.0%) rodent species and salivarian trypanosomes closely related to T. evansi were detected in Leopoldamys (20%) and Rattus (2.0%) species. Herpetosoma were prevalent among rodents associated with both human and sylvatic habitats, while three of the four salivaria-positive rodents were from a forest biotope. A Herpetosoma ITS-1 sequence amplified from one of these samples was 97.9% identical to that reported for T. lewisi in an experimentally infected rat and 96.4% identical to the sequence amplified from blood from a Thai infant. Habitats where rodents were collected significantly affect rodent infection, at least for T. lewisi, suggesting that the degree of anthropization may influence the transmission of Trypanosoma spp. These results suggest that multiple Herpetosoma species or strains are enzootic to Thailand, and that Rattus and Bandicota species are possible sources of human exposure to these parasites. (C) 2007 Published by Elsevier B.V.