Relaxant Effect of Sodium Tanshinone IIA Sulphonate on Mouse Tracheal Smooth Muscle
Relaxant Effect of Sodium Tanshinone IIA Sulphonate on Mouse Tracheal Smooth Muscle
复制标题
丹参酮IIA磺酸钠对小鼠气管平滑肌的松弛作用
DOI：
10.1055/s-0042-119950
复制
发表时间：
2016-12
期刊：
影响因子：
2.7
通讯作者：
Zhou Wen-Liang
中科院分区：
文献类型：
--
作者：
Zhang Yi-Lin;Xu Jia-Wen;Wu Xiong-Hao;Chen Peng-Xiao;Wei Fang;Gao Dong-Dong;Chen Xiao-Wen;Luo Yu-Li;Zhu Yun-Xin;Huang Jiehong;Tan Ya-Xia;Zhao Lei;Zhou Wen-Liang
摘要丹参酮IIA磺酸钠是丹参酮IIA的水溶性衍生物，已被证明具有多种生物学特性，但其对气管平滑肌的药理作用尚不清楚。本文研究了丹参酮IIA磺酸钠对小鼠气管平滑肌的松弛作用及其机制。用机械记录系统评价丹参酮IIA磺酸钠对小鼠气管环的松弛作用。用激光共聚焦成像系统测定原代培养的气管平滑肌细胞内钙离子浓度。结果表明，丹参酮ⅡA磺酸钠以β受体和上皮非依赖性的方式引起小鼠气管环的剂量依赖性松弛。用ATP敏感的K+通道阻断剂格列本脲预处理可部分减弱这种松弛反应。丹参酮IIA磺酸钠显著抑制细胞外钙离子引起的收缩。高钾或卡巴胆碱引起的细胞内钙离子浓度升高也可被丹参酮IIA磺酸钠阻断。结论：丹参酮ⅡA磺酸钠的松弛作用不依赖于β肾上腺素能受体和气道上皮细胞，其作用机制主要是通过L型电压依赖性钙通道抑制细胞外钙内流，部分通过激活ATP敏感性钾通道实现的。提示丹参酮IIA磺酸钠治疗哮喘具有潜在的治疗价值。
Abstract Sodium tanshinone IIA sulphonate, a water-soluble derivative of tanshinone IIA, has been proven to possess versatile biological properties, but its pharmacological effect on tracheal smooth muscle remains elusive. This paper presents a study on the relaxant effect and underlying mechanisms of sodium tanshinone IIA sulphonate on mouse tracheal smooth muscle. The relaxant effect of sodium tanshinone IIA sulphonate was evaluated in mouse tracheal rings using a mechanical recording system. Intracellular Ca2+ concentration was measured in primary cultured tracheal smooth muscle cells using confocal imaging system. The results showed that sodium tanshinone IIA sulphonate induced dose-dependent relaxation of mouse tracheal rings in a β-adrenoceptor- and epithelium-independent manner. Pretreatment with the ATP-sensitive K+ channel blocker glibenclamide partly attenuated the relaxation response. Administration of sodium tanshinone IIA sulphonate notably inhibited the extracellular Ca2+-induced contraction. High KCl or carbachol-evoked elevation in the intracellular Ca2+ concentration was also abrogated by sodium tanshinone IIA sulphonate in tracheal smooth muscle cells. In conclusion, the tracheal relaxant effect of sodium tanshinone IIA sulphonate was independent of β-adrenoceptor and airway epithelium, mediated primarily by inhibition of extracellular Ca2+ influx via L-type voltage-dependent Ca2+ channels and partially by activation of the ATP-sensitive K+ channel. These results indicate the potential therapeutic value of sodium tanshinone IIA sulphonate for asthma treatment.
登录
查看更多内容
影响因子：
9.1
作者：
Xu, Yan;Feng, Dechun;Xu, Lingyun
通讯作者：
Xu, Lingyun
DOI：
--
发表时间：
1998
期刊：
Lung biology in health and disease
影响因子：
--
作者：
R. Strunk;R. Nicklas;H. Milgrom;M. L. Davis;D. Ikle
通讯作者：
R. Strunk;R. Nicklas;H. Milgrom;M. L. Davis;D. Ikle
DOI：
--
发表时间：
1997
期刊：
--
影响因子：
--
作者：
魏赞美
通讯作者：
魏赞美
丹参酮IIA磺酸钠抑制肺动脉高压大鼠肺动脉平滑肌典型瞬时受体电位表达
DOI：
10.1165/rcmb.2012-0071oc
发表时间：
2013-01-01
影响因子：
6.4
作者：
Wang, Jian;Jiang, Qian;Lu, Wenju
通讯作者：
Lu, Wenju
影响因子：
13.8
作者：
I. Wessler;C. Kirkpatrick;K. Racké
通讯作者：
I. Wessler;C. Kirkpatrick;K. Racké