Utility of mycobacterial interspersed repetitive unit typing for differentiating multidrug-resistant Mycobacterium tuberculosis isolates of the Beijing family
Utility of mycobacterial interspersed repetitive unit typing for differentiating multidrug-resistant Mycobacterium tuberculosis isolates of the Beijing family
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DOI：
10.1128/jcm.43.1.306-313.2005
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发表时间：
2005-01-01
影响因子：
9.4
通讯作者：
van Soolingen, D
中科院分区：
文献类型：
--
作者：
Kam, KM;Yip, CW;van Soolingen, D
分枝杆菌散在重复单位（MIRU）分型已被发现允许快速，可靠，高通量的结核分枝杆菌基因分型，并可能代表一种可行的方法来研究全球M。结核病分子流行病学目的探讨MIRU分型在耐药支原体鉴别中的应用。对2000 ~ 2003年在香港收集的102株北京基因型结核病菌株、102株多药耐药（MDR）临床分离株和253株非MDR临床分离株进行12位点MIRU分型、spoligotyping和IS 6110限制性片段长度多态性（RFLP）分型。Spoligo分型结果显示，355株分离株中有243株（68.5%）属于北京科基因型。与IS 6110 RFLP方法（HGI = 0.9979）相比，MIRU分型在区分北京家系菌株方面显示出较低的区分度（Hunter-Gaston区分指数[HGI]为0.8827）。对于非北京菌株，MIRU分型提供的区分度（HGI = 0.9929）与RFLP方法（HGI = 0.9961）相当。两种方法在区分耐药株和非耐药株的能力上无显著差异。结核MIRU基因分型数据构建的树状图显示北京基因型和非北京基因型之间存在明显的分离。在MIRU分型基础上增加RFLP的区分能力（92.6%）高于在RFLP基础上增加MIRU分型的区分能力（40.0%）。这为该方法用于MDR M的遗传多样性分析提供了依据。结核菌株可能处于不同的进化分化水平。
Mycobacterial interspersed repetitive unit (MIRU) typing has been found to allow rapid, reliable, high-throughput genotyping of Mycobacterium tuberculosis and may represent a feasible approach to study global M. tuberculosis molecular epidemiology. To evaluate the use of MIRU typing in discriminating drug-resistant M. tuberculosis strains of the Beijing genotype family, 102 multidrug-resistant (MDR) clinical isolates and 253 randomly selected non-MDR isolates collected from 2000 to 2003 in Hong Kong were subjected to 12-locus MIRU typing, spoligotyping, and IS6110 restriction fragment length polymorphism (RFLP) typing. Spoligo-typing showed that 243 (68.5%) of 355 isolates belonged to Beijing family genotype. MIRU typing showed lower discrimination in differentiating between the Beijing family strains (Hunter-Gaston discriminative index [HGI] of 0.8827) compared with the IS6110 RFLP method (HGI = 0.9979). For non-Beijing strains, MIRU typing provided discrimination (HGI = 0.9929) comparable to that of the RFLP method (HGI = 0.9961). There was no remarkable difference in discrimination power between the two methods in differentiating both within and between MDR and non-MDR strains of M. tuberculosis. Dendrograms constructed with the MIRU typing data showed a clear segregation between the Beijing and non-Beijing genotype. Addition of RFLP to MIRU typing offered a higher discrimination ability (92.6%) than did addition of MIRU typing to RFLP (40.0%). This supported the potential use of this method to analyze the global genetic diversity of MDR M. tuberculosis strains that may be at different levels of evolutionary divergence.