Atomic absorption spectrometry analysis of trace elements in degenerated intervertebral disc tissue.
Atomic absorption spectrometry analysis of trace elements in degenerated intervertebral disc tissue.
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DOI：
10.12659/msm.890654
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发表时间：
2014-11-04
期刊：
影响因子：
--
通讯作者：
Gasik R
中科院分区：
文献类型：
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作者：
Kubaszewski Ł;Zioła-Frankowska A;Frankowski M;Nowakowski A;Czabak-Garbacz R;Kaczmarczyk J;Gasik R
人体椎间盘组织中微量元素（TE）的研究很少。微量元素的存在可以有多种含义：必需TE表示组织的代谢方式，而环境相关TE表示污染和组织特异性吸收和积累。IVD是一种高度特异性的腔室，与相邻骨的通信受损。对IVD中TE的分析提供了关于组织代谢和IVD与其他组织通信的新见解。从22例退行性疾病的手术治疗中获得30个椎间盘。采用原子吸收光谱法测定样品中Al、Cd、Pb、Cu、Ni、Mo、Mg、Zn的浓度。所有样品均检测到Al、Pb、Cu、Mg、Zn。铅浓度与患者年龄呈显著正相关，镍浓度与患者居住地人口数量呈弱相关。只有Cu在IVD中的浓度高于其他组织。Mg/Zn、Mg/Al、Mg/Pb、Zn/Al、Zn/Pb和Al/Pb之间呈显著正相关。Mg/Cd、Zn/Cd、Mg/Mo和Mo/Pb呈负相关。本研究是为数不多的对退行性改变患者椎间盘内因素进行分析的研究之一。我们报告了与其他组织相比，椎间盘中微量元素浓度的显著差异。TE积累模式的知识对于更好地理解椎间盘营养和代谢至关重要。
Few studies have investigated trace elements (TE) in human intervertebral disc (IVD) tissue. Trace element presence can have diverse meanings: essential TE show the metabolic modalities of the tissue, while environmentally-related TE indicate pollution and tissue-specific absorption and accumulation. IVD is a highly specific compartment with impaired communication with adjacent bone. Analysis of TE in IVD provides new insights regarding tissue metabolism and IVD communication with other tissues. Thirty intervertebral discs were acquired from 22 patients during surgical treatment for degenerative disease. Atomic absorption spectrometry was used to evaluate the concentrations of Al, Cd, Pb, Cu, Ni, Mo, Mg, and Zn. Al, Pb, Cu, Mg, and Zn were detected in all samples. Pb was significantly positively correlated with age, and Ni concentration was weakly correlated with population count in the patient’s place of residence. Only Cu was observed in higher concentrations in IVD compared to in other tissues. Significant positive correlations were observed between the following pairs: Mg/Zn, Mg/Al, Mg/Pb, Zn/Al, Zn/Pb, and Al/Pb. Negative correlations were observed between Mg/Cd, Zn/Cd, Mg/Mo, and Mo/Pb. This study is one of few to profile the elements in intervertebral discs in patients with degenerative changes. We report significant differences between trace element concentrations in intervertebral discs compared to in other tissues. Knowledge of the TE accumulation pattern is vital for better understanding intervertebral disc nutrition and metabolism.