Supplementary sampling of obturation materials enhances microbial analysis of endodontic treatment failures: a proof of principle study
Supplementary sampling of obturation materials enhances microbial analysis of endodontic treatment failures: a proof of principle study
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DOI：
10.1007/s00784-014-1231-4
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发表时间：
2015-03-01
影响因子：
3.4
通讯作者：
Al-Ahmad, A.
中科院分区：
文献类型：
--
作者：
Karygianni, L.;Anderson, A. C.;Al-Ahmad, A.
根管治疗失败往往与持续的生物材料相关的牙髓感染。本研究的目的是评估根管充填材料采样与治疗后根尖周炎对提高标准的研究protocols.Samples从以前填充的根管和相应的根管充填材料的影响，从5根填充牙齿与治疗后根尖周病变。培养后，对分离的微生物进行定量和生化鉴定。此外，从相同样品中扩增细菌16 S核糖体DNA（rDNA）后构建克隆文库。对来自所选克隆的DNA进行测序以鉴定微生物物种。透射电子显微镜（TEM）辅助可视化的检测bacteries.Overall，22类群的厚壁菌门，放线菌，拟杆菌门检测到在这两个闭塞和根管样本的培养依赖和文化独立的方法。根管充填物保护了17种细菌（3.30-7.50 × 10（3）CFU/ml）。其中，9例仅在回收的填塞材料中检测到。再感染根管中有13个菌群（3.48-7.36 × 10（3）CFU/ml）。专性和兼性厌氧菌占优势。在一个样本中，不同物种的数量从1到5不等。将根管充填材料的采样与标准根管样本采集相结合，可以更清楚地了解再感染根管的实际微生物植物群，并改进继发性/持续性根管感染的研究方案。
Root canal treatment failures often correlate with persistent biomaterial-associated endodontic infections. The aim of the present study was to assess the impact of endodontic obturation material sampling from root canals with posttreatment apical periodontitis on improving standard study protocols.Samples from previously filled root canals and their corresponding endodontic filling materials were obtained from five root-filled teeth with posttreatment periradicular lesions. After cultivation, the isolated microorganisms were quantified and biochemically identified. Moreover, clone libraries were constructed after the amplification of bacterial 16S ribosomal DNA (rDNA) from the same samples. DNA from selected clones was sequenced to identify microbial species. Transmission electron microscopy (TEM) aided visualization of the detected bacteria.Overall, 22 taxa of the phyla Firmicutes, Actinobacteria, and Bacteroidetes were detected in both obturation and root canal samples by culture-dependent and culture-independent methods. Root canal fillings sheltered 17 species (3.30-7.50 x 10(3) CFU/ml). Of these, nine were detected solely in the retrieved obturation materials. The reinfected root canals harbored 13 taxa (3.48-7.36 x 10(3) CFU/ml). Obligate and facultative anaerobic bacteria prevailed. The number of different species ranged from 1 to 5 within a single sample. Fungi were not detected.Bacteria can colonize both root canals and endodontic fillings in vivo.Integrating the sampling of obturation materials with standard root canal sample collection offers a clearer insight into the actual microbial flora of reinfected root canals and improves the study protocols of secondary/persistent endodontic infections.