Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering
Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering
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DOI：
10.1016/s1010-7940(00)00373-0
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发表时间：
2000-05-01
影响因子：
3.4
通讯作者：
Turina, M
中科院分区：
文献类型：
--
作者：
Ye, Q;Zünd, G;Turina, M
目的：在组织工程中，三维生物可降解支架通常被用作细胞锚定、细胞增殖和细胞分化的基本结构。目前在心血管组织工程中使用的可生物降解支架具有潜在的免疫原性，它们表现出毒性降解和炎症反应。本研究的目的是建立一种新的细胞内三维培养体系，实现细胞内分布均匀、组织快速发育。而且没有毒性降解或炎症反应。方法：取手术室人升主动脉组织，培养成纯人肌成纤维细胞。将培养的人肌成纤维细胞悬浮在纤维蛋白原溶液中，接种于6孔培养板中培养4周，并添加不同浓度的抑肽酶。羟脯氨酸测定和组织学研究评估了这些纤维蛋白凝胶结构的组织发育情况。结果：基于三维纤维蛋白凝胶结构的组织发育的光镜和透射电子显微镜研究显示，细胞生长均匀，胶原合成融合。未检测到毒性降解或炎症反应。此外，在不加抑肽酶的培养液中，纤维蛋白凝胶肌成纤维细胞的结构在2d内溶解，但添加较高浓度的抑肽酶的培养液保留了三维结构，并具有更高的胶原含量(P<；0.005)和更好的组织发育。结论：三维纤维蛋白凝胶结构具有可控降解、良好的种植效果和良好的组织发育能力，可作为组织工程支架材料。(C)2000 Elsevier Science B.V.保留所有权利。
Objective: In tissue engineering, three-dimensional biodegradable scaffolds are generally used as a basic structure for cell anchorage, cell proliferation and cell differentiation. The currently used biodegradable scaffolds in cardiovascular tissue engineering are potentially immunogenic, they show toxic degradation and inflammatory reactions. The aim of this study is to establish a new three-dimensional cell culture system within cells achieve uniform distribution and quick tissue development. and with no toxic degradation or inflammatory reactions. Methods: Human aortic tissue is harvested from the ascending aorta in the operation room and worked up to pure human myofibroblasts cultures. These human myofibroblasts cultures are suspended in fibrinogen solution and seeded into 6-well culture plates for cell development for 4 weeks and supplemented with different concentrations of aprotinin. Hydroxyproline assay and histological studies were performed to evaluate the tissue development in these fibrin gel structures. Results: The light microscopy and the transmission electron microscopy studies for tissue development based on the three-dimensional fibrin gel structures showed homogenous cell growth and confluent collagen production. No toxic degradation or inflammatory reactions could be detected. Furthermore, fibrin gel myofibroblasts structures dissolved within 2 days in medium without aprotinin, but medium supplemented with higher concentration of aprotinin retained the three-dimensional structure and had a higher collagen content (P < 0.005) and a better tissue development. Conclusions: A three-dimensional fibrin gel structure can serve as a useful scaffold for tissue engineering with controlled degradation, excellent seeding effects and good tissue development. (C) 2000 Elsevier Science B.V. All rights reserved.