Olfactory GTP-binding protein: signal-transducing polypeptide of vertebrate chemosensory neurons.
Olfactory GTP-binding protein: signal-transducing polypeptide of vertebrate chemosensory neurons.
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嗅觉 GTP 结合蛋白：脊椎动物化学感应神经元的信号转导多肽。
DOI：
10.1073/pnas.83.13.4947
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发表时间：
1986
影响因子：
11.1
通讯作者：
Lancet,D
中科院分区：
文献类型：
--
作者：
Pace,U;Lancet,D
嗅觉涉及通过气味刺激感觉神经元以产生去极化和动作电位。我们表明，嗅觉反应可能是由GTP结合蛋白（G蛋白），一个同系物的视觉，激素和大脑信号转导多肽介导的。嗅觉G蛋白在三种脊椎动物的化学感觉神经元的分离的树突状膜（嗅觉纤毛制剂）中被鉴定，并且显示出介导这些膜中的高活性腺苷酸环化酶的气味剂的刺激。嗅觉神经元的G蛋白与激素刺激性GTP结合蛋白Gs最相似。它的α亚基的分子量约为42，000，它经历霍乱毒素催化的ADP-核糖基化，导致腺苷酸环化酶活化。青蛙嗅觉和肝脏Gs α亚基的分子量略有不同，嗅觉腺苷酸环化酶对非水解GTP类似物的敏感性更高，这与可能存在不同的Gs变体是一致的。嗅觉G蛋白引起的信号放大可能是嗅觉异常灵敏的原因。
The sense of smell involves the stimulation of sensory neurons by odorants to produce depolarization and action potentials. We show that olfactory responses may be mediated by a GTP-binding protein (G protein), a homolog of the visual, hormonal, and brain signal transducing polypeptides. The olfactory G protein is identified in isolated dendritic membranes (olfactory cilia preparations) of chemosensory neurons from three vertebrate species and is shown to mediate the stimulation by odorants of the highly active adenylate cyclase in these membranes. The G protein of olfactory neurons is most similar to Gs, the hormonal stimulatory GTP-binding protein. Its alpha subunit has a molecular weight of about 42,000, and it undergoes ADP-ribosylation catalyzed by cholera toxin that leads to adenylate cyclase activation. The slight difference in molecular weights of the frog olfactory and the liver Gs alpha subunits and the higher sensitivity of olfactory adenylate cyclase to nonhydrolyzable GTP analogs are consistent with the possible existence of different Gs variants. Signal amplification due to the olfactory G protein may be responsible for the unusual acuity of the sense of smell.