Molecular cloning and characterization of the gene encoding osmotin protein in Petunia hybrida
Molecular cloning and characterization of the gene encoding osmotin protein in Petunia hybrida
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DOI：
10.1016/s0168-9452(02)00016-x
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发表时间：
2002-05-01
期刊：
影响因子：
5.2
通讯作者：
Kim, SG
中科院分区：
文献类型：
--
作者：
Kim, H;Mun, JH;Kim, SG
从矮牵牛花瓣原生质体培养的cDNA文库中分离到一个渗透蛋白基因克隆PhOSM。PhOSM基因由一个开放阅读框组成，编码246个氨基酸，计算出其相对分子质量为26.7 kDa。数据库比较显示，PhOSM蛋白序列与烟草渗透素和烟草OLP(渗透蛋白样蛋白)有很高的同源性(89%)。3，4-脱氢-L-脯氨酸能完全抑制花瓣原生质体培养物中PhOSM mRNA的积累，DHP是细胞壁再生中肽-脯氨酸羟化的抑制剂。RNA印迹分析表明，PhOSM在开花后3d主要在根中表达，在雌蕊中有少量表达。真菌青霉菌、斯氏欧文氏菌和紫丁香假单胞菌对PhOSM的诱导作用较强，而对网状曲霉的诱导作用较弱。在伤害后，PhOSM转录本在直接受损的叶片中被诱导，但不是系统地诱导。此外，PhOSM转录水平随着十八烷类途径中间体以及阿司匹林和水杨酸处理的增加而增加。我们的结果表明，PhOSM不仅参与创伤应激信号转导，而且在发育过程中受到调节。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
A cDNA clone encoding osmotin, PhOSM, was isolated from a cDNA library constructed from petal protoplast cultures of Petunia hybrida. PhOSM cDNA was composed of an open reading frame corresponding to protein of 246 amino acids and had a calculated molecular weight of 26.7 kDa. Database comparisons of the PhOSM protein sequences revealed high identity (89%) with the tobacco osmotin and tobacco OLP (Osmotin-Like-Protein). The increased accumulation of PhOSM mRNA in petal protoplast cultures was completely inhibited by treatment with Dhp (3,4-dehydro-L-proline), which is a inhibitor of peptydyl proline hydroxylation in cell wall regeneration. RNA blot analyses revealed that PhOSM was expressed primarily in roots and slightly in the pistil, 3 days after flowering. PhOSM expression was strongly induced in leaves that were exposed to Penicillium funiculosum, Erwinia stewartii and Pseudomonas syringae but not to Aspergillus nidulans. Upon wounding, PhOSM transcripts were induced in the directly damaged leaf but not systemically. Moreover, PhOSM transcript levels increased in response to octadecanoid pathway intermediates and treatments with aspirin and salicylic acid. Our results indicate that PhOSM is developmentally regulated as well as involved in wound-stress signal transduction. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.