Dynamic nutrient acquisition from a hydrated apoplast supports biotrophic proliferation of a bacterial pathogen of maize
Dynamic nutrient acquisition from a hydrated apoplast supports biotrophic proliferation of a bacterial pathogen of maize
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DOI：
10.1016/j.chom.2022.03.017
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发表时间：
2022-04-13
影响因子：
30.3
通讯作者：
Mackey, David
中科院分区：
文献类型：
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作者：
Gentzel, Irene;Giese, Laura;Mackey, David
植物病原体扰乱寄主，创造适合其繁殖的环境，包括抑制免疫，促进水分和养分的可获得性。尽管坏死菌通过破坏宿主细胞的完整性来获得水分和营养物质，但半营养菌是否如细菌病原菌Pantoea stewartii亚种尚不清楚。Stewartii(Pnss)，在感染的早期生物营养阶段主动释放水分和营养物质。在这里，我们表明，水分和代谢产物积累在侵染Pnss的玉米叶片的质外体中，先于宿主细胞完整性的破坏。在这个生物营养阶段，营养物质的获取是一个动态的过程；代谢产物进入质外体的速度限制了它们被PNSS细胞增殖同化的速度。由Avre家族III型效应器WtsE驱动的水合和营养质外体的形成。鉴于Avre家族效应器的广泛分布，这项工作强调了在生物营养过程中积极获取水和营养物质对植物病原细菌增殖的重要性。
Plant pathogens perturb their hosts to create environments suitable for their proliferation, including the suppression of immunity and promotion of water and nutrient availability. Although necrotrophs obtain water and nutrients by disrupting host-cell integrity, it is unknown whether hemibiotrophs, such as the bacterial pathogen Pantoea stewartii subsp. stewartii (Pnss), actively liberate water and nutrients during the early, biotrophic phase of infection. Here, we show that water and metabolite accumulation in the apoplast of Pnss-infected maize leaves precedes the disruption of host-cell integrity. Nutrient acquisition during this biotrophic phase is a dynamic process; the partitioning of metabolites into the apoplast rate limiting for their assimilation by proliferating Pnss cells. The formation of a hydrated and nutritive apoplast is driven by an AvrE-family type III effector, WtsE. Given the broad distribution of AvrE-family effectors, this work highlights the importance of actively acquiring water and nutrients for the proliferation of phytopathogenic bacteria during biotrophy.