Fluorescence properties of albumin blue 633 and 670 in plasma and whole blood
Fluorescence properties of albumin blue 633 and 670 in plasma and whole blood
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DOI：
10.1117/1.1381053
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发表时间：
2001-07-01
影响因子：
3.5
通讯作者：
Lakowicz, JR
中科院分区：
文献类型：
--
作者：
Abugo, OO;Herman, P;Lakowicz, JR
我们已经测定了两种长波长染料，即白蛋白蓝633（AB633）和670（AB670）在血浆和血液中的荧光特性，以评估在血液中进行直接荧光传感测量的可能性。通过结合和寿命测量，我们还能够表明这些染料在血浆和血液中选择性地与人血清白蛋白（HSA）结合。通过测量AB670平均寿命随HSA浓度的变化，我们表明基于寿命的传感可用于使用这些白蛋白蓝染料监测HSA浓度。对血浆和血液中AB633和AB670的各向异性测量显示，这些染料在这些介质中具有较高的各向异性值。利用这些高各向异性，我们还能够通过AB670各向异性随HSA浓度的变化来确定血浆和血液模拟物中的HSA浓度。这些结果表明，除了能够直接在血浆和血液中进行荧光测量外，还可以使用优先与特定血浆/血液成分结合的荧光探针直接对其进行传感。（C）2001年美国光电仪器工程师协会。
We have determined the fluorescence characteristics of two long wavelength dyes, albumin blue 633 (AB633) and 670 (AB670), in plasma and blood to evaluate the possibility of making direct fluorescence sensing measurements in blood. Using binding and lifetime measurements we were also able to show that these dyes bind selectively to human serum albumin (HSA) in plasma and blood. By measuring changes in the mean lifetime of AB670 with changes in the HSA concentration, we showed that lifetime-based sensing can be used to monitor HSA concentrations using these albumin blue dyes. Anisotropy measurements for AB633 and AB670 in plasma and blood revealed high anisotropy values for these dyes in these media. Exploiting these high anisotropies, we were also able to determine HSA concentrations in plasma and blood mimics using changes in AB670 anisotropy with HSA concentration. These results show that, apart from being able to make fluorescence measurements directly in plasma and blood, it is possible to sense directly for specific plasma/blood components using fluorescent probes that bind preferentially to them. (C) 2001 Society of Photo-Optical Instrumentation Engineers.