Toxicity of a synthetic phenolic antioxidant, butyl hydroxytoluene (BHT), in vertebrate model zebrafish embryo ( Danio rerio )
Toxicity of a synthetic phenolic antioxidant, butyl hydroxytoluene (BHT), in vertebrate model zebrafish embryo ( Danio rerio )
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DOI：
10.1111/are.14732
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发表时间：
2020-09
影响因子：
2
通讯作者：
Raktim Sarmah;Sarada Kanta Bhagabati;R. Dutta;Dipanka Nath;H. Pokhrel;L. Mudoi;Nishi Sarmah;Jiten Sarma;A. M. Ahmed;Rupam Jyoti Nath;Lucy Ingtipi;Kedolhouse Kuotsu
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作者：
Raktim Sarmah;Sarada Kanta Bhagabati;R. Dutta;Dipanka Nath;H. Pokhrel;L. Mudoi;Nishi Sarmah;Jiten Sarma;A. M. Ahmed;Rupam Jyoti Nath;Lucy Ingtipi;Kedolhouse Kuotsu
合成抗氧化剂3,5 -二叔丁基- 4 -羟基甲苯（BHT）被广泛用作食品、化妆品和塑料工业的添加剂，以提高食品和塑料的可持续性。在哺乳动物体内，以及在河流和水中等自然环境中，BHT可降解为3,5‐二甲基丁基‐4‐羟基苯甲醛（BHT‐CHO）。据估计，人类每天对BHT的平均摄入量为每公斤体重0.3毫克。尽管它被认为在规定的水平上对人体是安全的，但它在水生环境中的普遍存在和有争议的毒理学数据引起了人类和水生生物的极大关注。斑马鱼胚胎毒性试验（ZFET）的实验结果表明，胚胎期暴露96小时的急性毒性（LC50）为4.388 mg/L，有效浓度（EC50）为1.375 mg/L。亚致死浓度导致的心率降低表明该化学品对心脏有毒，但需要进一步审查。计算所得的致畸指数（TI）为3.19，表明该化合物可能是水生生物的潜在致畸物。本研究的发现将为BHT的发育毒性提供更多的证据，这对进一步的生态毒理学研究的风险评估具有重要意义。
The synthetic antioxidant 3,5‐di‐tert‐butyl‐4‐hydroxytoluene (BHT) is widely used as an additive in the food, cosmetic and plastic industries to increase the tenability of food and plastic for the past 70 years. BHT is degraded to 3,5‐di‐test‐butyl‐4‐hydroxybenzaldehyde (BHT‐CHO) in mammals, as well as in the natural environment such as in river and water. The average daily intake of BHT for human being is estimated to be 0.3 mg/kg body weight. Even though it is considered safe for human at authorized level, but its ubiquitous presence in the aquatic environment and the controversial toxicological data are of great concern for human as well as aquatic life. The experimental findings of zebrafish embryo toxicity test (ZFET) showed that the acute toxicity of 96‐hr (LC50) exposure during the embryogenic stage was found to be 4.388 mg/L and the effective concentration (EC50) was 1.375 mg/L. The reduce heart rate from the sublethal concentrations indicates the chemical to be cardiotoxic but a further review is to be needed. The Teratogenic Index (TI) calculated to be 3.19, which implies the compound may be a potential teratogen in aquatic life. The findings obtained in this study will stretch more evidence regarding developmental toxicity of BHT, which will be of much importance in further risk assessment of ecotoxicological studies.