WSV152 induces apoptosis and promotes viral replication in Litopenaeus vannamei
WSV152 induces apoptosis and promotes viral replication in Litopenaeus vannamei
复制标题
WSV152 诱导凡纳滨对虾细胞凋亡并促进病毒复制
DOI：
10.1016/j.fsi.2020.01.020
复制
发表时间：
2020
影响因子：
4.7
通讯作者：
Gong Han
中科院分区：
文献类型：
--
作者：
Yan Muting;Liu Zhihao;Xu Kaihang;Wang Wenjing;Fan Lanfen;Gong Han
Previous studies have indicated that white spot syndrome virus (WSSV) infection induces apoptosis in many shrimp organs. However, the mechanism by which WSSV causes host apoptosis remains largely unknown. In this study, we demonstrated the function of wsv152, the first mitochondrial protein identified as encoded by WSSV. Glutathione S-transferase pulldown and co-immunoprecipitation analysis revealed that wsv152 interacts with the shrimp mitochondrial protein cytochromecoxidase 5a (COX5a), a subunit of the COX complex. We also found that wsv152 expression significantly increased the rate of apoptosis, suggesting a role of wsv152 in WSSV-induced apoptosis in shrimp. Knockdown of wsv152in vivoled to downregulation of several apoptosis-related shrimp genes, including cytochromec, apoptosis-inducing factor and caspase-3. Suppression of wsv152 also resulted in significant reductions in the number of WSSV genome copies in tissues and in the mortality of WSSV-infected shrimp. Together, these results suggest that wsv152 targets host COX5a and is associated with the expression profiles of apoptosis-related shrimp genes. Wsv152 is likely also involved in WSSV-induced apoptosis, thereby facilitating virus infection and playing a complex role in WSSV pathogenesis.
