Gene expression phenotypes for lipid metabolism and intramuscular fat in skeletal muscle of cattle
Gene expression phenotypes for lipid metabolism and intramuscular fat in skeletal muscle of cattle
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DOI：
10.2527/jas.2012-5409
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发表时间：
2013-03-01
影响因子：
3.3
通讯作者：
Dalrymple, B. P.
中科院分区：
文献类型：
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作者：
De Jager, N.;Hudson, N. J.;Dalrymple, B. P.
评估牛骨骼肌肌内脂肪（IMF）的基因表达表型，以替代传统的IMF%估计值。从LM发展的时间过程中的基因表达数据在高和低大理石花纹的普通牛牛杂交进行了比较，以确定基因参与肌内脂肪细胞脂质代谢与发育相似的基因表达谱。鉴定了三组基因：三酰甘油（TAG）合成和储存，脂肪酸（FA）合成和PPAR。相关基因在对48头印度牛的LM进行的独立分析中，TAG和FA基因集富集在前100个基因中，其中表达与IMF%最相关（P分别= 1.2 x 10（-24）和3.5 x 10（-9））。一般而言，编码肌内脂肪细胞中参与FA和TAG合成的酶的基因存在于前100个基因中。在B。为了研究生长促进剂（HGP）、新南威尔士州（NSW）和西澳大利亚州（WA）这两个试验地点以及3种嫩度基因型对TAG基因组中基因表达水平的影响以及基因表达与IMF%的相关性。尽管在两个实验地点的HGP处理的动物中观察到12个单独TAG基因的表达与IMF%之间的相关性（平均r = 0.43），但在NSW地点的未处理动物中未观察到相关性（平均r =-0.07，P <3x 10（6））。然而，TAG基因显示，相对于未处理的牛，HGP处理的牛的LM中的表达平均降低1.6倍（P < 0.0004），这在两个实验地点之间是一致的。与WA（1.2倍减少，P = 0.03）相比，具有有利嫩度钙蛋白酶1和3以及钙蛋白酶抑制蛋白等位基因的牛在NSW（1.8倍减少，P = 0.0002）中的表达减少更大（P = 0.008）。TAG基因型对IMF%与TAG基因的相关性无显著影响（P > 0.05）。一般而言，基因型、处理和位置以及TAG基因集基因表达之间的相互作用与使用255只动物检测到的相同因素和IMF%之间的相互作用一致，其中本研究中的48只动物是一个子集。因此，TAG基因组构成了能够使用比当前方法小得多的组预测不同基因型和处理对IMF%的影响的基因表达表型，即使在具有非常低的IMF%的动物中。(C)2013年美国动物科学学会。All rights reserved.
Gene expression phenotypes were evaluated for intramuscular fat (IMF) in bovine skeletal muscle as an alternative to traditional estimates of IMF%. Gene expression data from a time course of LM development in high- and low-marbling Bos taurus cattle crosses were compared to identify genes involved in intramuscular adipocyte lipid metabolism with developmentally similar gene expression profiles. Three sets of genes were identified: triacylglyceride (TAG) synthesis and storage, fatty acid (FA) synthesis, and PPAR.-related genes. In an independent analysis in the LM of 48 Bos indicus cattle, TAG and FA gene sets were enriched in the top 100 genes of which expression was most correlated with IMF% (P = 1.2 x 10(-24) and 3.5 x 10(-9), respectively). In general, genes encoding enzymes involved in the synthesis of FA and TAG in the intramuscular adipocytes were present in the top 100 genes. In B. indicus, effects of a steroid hormone growth promotant (HGP), 2 experimental sites [New South Wales (NSW) and Western Australia (WA)], and 3 tenderness genotypes on the expression levels of genes in the TAG gene set and the correlation of gene expression with IMF% were investigated. Although correlation between expression of 12 individual TAG genes and IMF% was observed in HGP-treated animals in both experimental sites (mean r = 0.43), correlation was not observed for untreated animals at the NSW site (mean r = -0.07, P < 3 x 10 (6)). However, TAG genes showed an average 1.6-fold (P < 0.0004) reduction in expression in the LM of HGP-treated cattle relative to untreated cattle, an effect consistent across both experimental sites. Cattle possessing the favored tenderness calpain 1 and 3 and calpastatin alleles exhibited a greater (P = 0.008) reduction in expression in NSW (1.8-fold reduction, P = 0.0002) compared with WA (1.2-fold reduction, P = 0.03). Tenderness genotype had no impact (P > 0.05) on the correlation of TAG genes with IMF%. In general, the interactions among genotype, treatment and location, and TAG gene set gene expression were consistent with the interactions among the same factors and IMF% detected using 255 animals, of which the 48 in this study were a subset. Thus, the TAG gene set constitutes a gene expression phenotype able to predict effects of different genotypes and treatments on IMF% using much smaller groups than current approaches, even in animals with very low IMF%. (C) 2013 American Society of Animal Science. All rights reserved.