Multi-gene phylogenies and phenotypic characters distinguish two species within the Colletogloeopsis zuluensis complex associated with Eucalyptus stem cankers
Multi-gene phylogenies and phenotypic characters distinguish two species within the Colletogloeopsis zuluensis complex associated with Eucalyptus stem cankers
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DOI：
10.3114/sim.55.1.133
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发表时间：
2006-01-01
影响因子：
16.5
通讯作者：
Wingfield, Michael J.
中科院分区：
文献类型：
--
作者：
Cortinas, Maria-Noel;Crous, Pedro W.;Wingfield, Michael J.
Colletogloeopsis zuluensis，以前被称为Coniothyrium zuluense，在许多热带和亚热带国家作为非本地种植的桉树种上引起严重的茎溃疡病。这种茎溃疡病首先在南非报告，随后在其他非洲国家以及南美洲和东南亚国家的各种桉树和杂交桉树上发现了这种疾病。在以往的研究中，基于ITS区DNA序列数据的系统发育分析表明，祖鲁ensis的所有物质都是单系的。然而，真菌在更多国家的出现，以及对其他分离物的DNA序列分析，挑战了单一物种与Coniothyrium canker有关的概念。本研究的目的是考虑在所有可用地点分离的祖鲁弧菌之间的系统发育关系，并通过表型和形态比较来支持这些分析。利用ITS区域的DNA序列、p -微管蛋白基因的3 - 6外显子、翻译延伸因子1- α基因的内含子和线粒体atp酶6基因的部分序列构建了个体和组合的系统发育。系统发育数据和形态特征都清楚地表明，该菌株至少代表两个分类群。其中之一是祖鲁猿猴，因为它最初是在南非被描述的，我们为它提供了一个表型。第二种出现在阿根廷和乌拉圭，最近被描述为高chensis。这两种真菌都是导致相同症状的严重病原体。将它们视为不同的物种具有重要的检疫后果。
Colletogloeopsis zuluensis, previously known as Coniothyrium zuluense, causes a serious stem canker disease on Eucalyptus spp. grown as non-natives in many tropical and sub-tropical countries. This stem canker disease was first reported from South Africa and it has subsequently been found on various species and hybrids of Eucalyptus in other African countries as well as in countries of South America and South-East Asia. In previous studies, phylogenetic analyses based on DNA sequence data of the ITS region suggested that all material of C. zuluensis was monophyletic. However, the occurrence of the fungus in a greater number of countries, and analyses of DNA sequences with additional isolates has challenged the notion that a single species is involved with Coniothyrium canker. The aim of this study was to consider the phylogenetic relationships amongst C. zuluensis isolates from all available locations and to support these analyses with phenotypic and morphological comparisons. Individual and combined phylogenies were constructed using DNA sequences from the ITS region, exons 3 through 6 of the P-tubulin gene, the intron of the translation elongation factor 1-alpha gene, and a partial sequence of the mitochondrial ATPase 6 gene. Both phylogenetic data and morphological characteristics showed clearly that isolates of C. zuluensis represent at least two taxa. One of these is C. zuluensis as it was originally described from South Africa, and we provide an epitype for it. The second species occurs in Argentina and Uruguay, and is newly described as C. gauchensis. Both fungi are serious pathogens resulting in identical symptoms. Recognising them as different species has important quarantine consequences.