Clinical follow-up in the rat experimental model of African-Trypanosomiasis
Clinical follow-up in the rat experimental model of African-Trypanosomiasis
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DOI：
10.1177/153537020322801114
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发表时间：
2003-12-01
影响因子：
3.2
通讯作者：
Buguet, A
中科院分区：
文献类型：
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作者：
Darsaud, A;Bourdon, L;Buguet, A
人类非洲锥虫（HAT）的动物模型已经开发出来，以了解导致进入神经阶段的致病机制，其中大部分是指组织学方面，但不是临床或行为数据。我们的研究旨在确定简单的临床和/或行为标志物之间的血淋巴相和脑膜脑炎阶段的疾病。用布氏锥虫AnTat 1.1 E.从感染当天至死亡，每天测量摄食量和体重。每周测量两次红细胞压积。通过旷场试验评价行为障碍。在感染后的第十二天，由于两天前开始的食物摄入量显著下降，体重突然减轻。红细胞压积测量显示大鼠出现贫血状态。旷场试验表明，它们在感染后第二周就不那么活跃和反应。一项补充的组织学研究在同一时期观察到脉络丛中的锥虫和炎性细胞。这些结果支持中枢神经系统功能障碍。所观察到的体重减轻被讨论为进入脑膜脑炎阶段的参数。大鼠模型再现了在人类疾病中观察到的神经系统症状，并可能被证明可用于进一步的神经组织学和治疗研究。
Animal models of Human African Trypanosomiasis (HAT) have been developed to understand the pathogenic mechanisms leading to the passage into the neurological phase, most of them referring to histological aspects but not clinical or behavioral data. Our study aimed at defining simple clinical and/or behavioral markers of the passage between the hemolymphatic phase and the meningo-encephalitic stage of the disease. Sprague-Dawley rats (n = 24) were infected with Trypanosoma brucei brucei AnTat 1.1 E. Food intake and body weight were measured daily from the day of infection until death. Hematocrit was measured twice a week. Behavioral disturbances were evaluated through an Open-field test. A sudden weight loss occurred on the twelfth day after infection, due to a significant drop of food intake starting two days before. The rats developed an anemic state shown by the hematocrit measurements. The Open-field test showed them to be less active and reactive as soon as the second week after infestation. A complementary histological study observed trypanosomes and inflammatory cells in the choroid plexus at the same period. These results are in favor of central nervous system functional disturbances. The observed weight loss is discussed as being a parameter of the entry in the meningo-encephalitic phase. The rat model reproduces neurological symptoms observed in the human disease and may prove to be useful for further neurohistological and therapeutic studies.