Identification of putative miRNA involved in Drosophila melanogaster immune response
Identification of putative miRNA involved in Drosophila melanogaster immune response
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DOI：
10.1016/j.dci.2011.03.034
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发表时间：
2012-02-01
影响因子：
2.9
通讯作者：
Lee, So Young
中科院分区：
文献类型：
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作者：
Fullaondo, Ane;Lee, So Young
MicroRNAs(MiRNAs)是真核生物中转录后调节基因表达的内源性非编码小RNA。众所周知，它们在体内平衡、发育、癌症和免疫反应等生理过程中发挥着不同的作用。在果蝇中，已鉴定出多达176个miRNAs；然而，它们的生物学功能仍不清楚。在这里，我们描述了一种电子筛选策略来识别参与特定免疫信号通路的miRNAs，该策略基于：(I)miRNAs针对给定路径Nay的mRNAs的潜在能力；(Ii)miRNAs跨物种的序列保守性；(Iii)miRNAs的表达谱。使用这一策略，我们定义了可能参与免疫反应的七个果蝇miRNAs的子集。有趣的是，其中一些miRNAs的靶标是肽聚糖受体蛋白(PG RN)，但目前尚不清楚其调节因子。MiRNA介导的调控可能解释了PGRPs是如何在免疫信号通路中受到控制的。(C)2011爱思唯尔有限公司。保留所有权利。
MicroRNAs (miRNAs) are small endogenous non-coding RNAs that post-transcriptionally regulate gene expression in eukaryotes. They are known to play diverse roles in physiological processes such as homeostasis, development, cancer and immune response. In Drosophila melanogaster up to 176 miRNAs have been identified; yet, their biological functions remain unknown. Here, we describe an in silico screening strategy to identify miRNAs involved in a specific immune signaling pathway that is based on: (i) the potential capability of miRNAs to target mRNAs of a given path Nay; (ii) the sequence conservation of miRNAs across species and (iii) the expression profile of miRNAs. Using this strategy, we have defined a subset of seven Drosophila miRNAs that are likely to participate in the immune response. Interestingly, some of these miRNAs target peptidoglycan receptor proteins (PG RN) for which no regulators are known yet. miRNA-mediated regulation may explain how PGRPs are controlled in the immune signaling pathway. (C) 2011 Elsevier Ltd. All rights reserved.