The plasma peptides of ovarian cancer
The plasma peptides of ovarian cancer
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DOI：
10.1186/s12014-018-9215-z
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发表时间：
2018-12-21
影响因子：
3.8
通讯作者：
Marshall, John G.
中科院分区：
文献类型：
--
作者：
Dufresne, Jaimie;Bowden, Pete;Marshall, John G.
背景利用液相色谱和串联质谱对不同临床人群血浆中的肽进行鉴定、定量和比较，有可能从血浆中发现新的诊断或治疗肽或蛋白。卵巢癌血浆的内源性胰蛋白酶肽进行了比较，乳腺癌和女性癌症正常对照，其他疾病与他们的匹配或正常对照，加上冰冷的血浆，以控制预分析variation.MethodsThe内源性胰蛋白酶肽或胰蛋白酶磷酸肽（即没有外源性消化）进行了分析，从200升EDTA血浆。血浆肽经差速离心有机/水梯度提取、干燥、C18收集后，用线性四极杆离子阱进行高效液相色谱-纳米电喷雾串联质谱（LC-ESI-MS/MS）分析。将卵巢癌的内源性肽与来自不同机构的多种疾病和正常样品以及冰冷对照进行比较。通过LC-ESI-MS/MS对肽进行随机和独立取样。通过SEQUEST和X！TANDEM算法，在SQL Server中过滤，然后用卡方检验（（2））进行频率计数，用STRING算法进行分析，并在R统计系统中用Tukey-Kramer Honestly Significant Difference（HSD）检验比较前体强度。和SERPINA 1在卵巢癌中显示增加的观察频率和/或前体强度。经卡方检验，许多细胞蛋白质的频率变化较大（（2）>60，p
BackgroundIt may be possible to discover new diagnostic or therapeutic peptides or proteins from blood plasma by using liquid chromatography andtandem mass spectrometryto identify, quantify and compare the peptides cleaved ex vivo from different clinical populations. The endogenous tryptic peptides of ovarian cancer plasma were compared to breast cancer and female cancer normal controls, other diseases with their matched or normal controls, plus ice cold plasma to control for pre-analytical variation.MethodsThe endogenous tryptic peptides or tryptic phospho peptides (i.e. without exogenous digestion) were analyzed from 200l of EDTA plasma. The plasma peptides were extracted by a step gradient of organic/water withdifferential centrifugation, dried, and collected over C18 for analytical HPLC nano electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS)with a linear quadrupole ion trap. The endogenous peptides of ovarian cancer were compared to multiple disease and normal samples from different institutions alongside ice cold controls. Peptides were randomly and independently sampled by LC-ESI-MS/MS. Precursor ions from peptides >E4 counts were identified by theSEQUEST and X!TANDEMalgorithms, filtered in SQL Server, before testing of frequency counts by Chi Square ((2)), foranalysiswith the STRING algorithm,and comparison of precursor intensity by ANOVA in the R statistical systemwith theTukey-Kramer Honestly Significant Difference (HSD) test.ResultsPeptides and/or phosphopeptides of common plasma proteins such as HPR, HP, HPX, and SERPINA1 showed increased observation frequency and/or precursor intensity in ovarian cancer. Many cellular proteins showed large changes in frequency by Chi Square ((2)>60, p