STRUCTURE AND COMPARISON OF THE OXYTOCIN AND VASOPRESSIN GENES FROM RAT
STRUCTURE AND COMPARISON OF THE OXYTOCIN AND VASOPRESSIN GENES FROM RAT
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DOI：
10.1073/pnas.81.7.2006
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
RICHTER, D
中科院分区：
文献类型：
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作者：
IVELL, R;RICHTER, D
从大鼠基因组文库中分离出编码催产素及其相关neurophysin的前体蛋白的基因，并测定了其序列。小基因（. apprxeq. 850个碱基对）预测的mRNA。500个碱基[没有poly（A）尾]。外显子-内含子结构与加压素基因相似，在蛋白质编码区有2个剪接位点。第一外显子（A）包含5“非编码启动子区、推定的信号肽、九肽激素催产素和NH 2末端、neurophysin可变区。第2外显子（B）编码neurophysin的中心保守区域，第3外显子（C）编码neurophysin的剩余COOH末端，在其末端有一个额外的精氨酸残基，推测在翻译后加工过程中被切割掉。外显子B内的一段143个核苷酸，除了一个碱基的变化，是完全同源的大鼠加压素基因的等效部分，提供支持的基因转换事件最近影响了2个基因。
The gene encoding the precursor protein to the hormone oxytocin and its associated neurophysin was isolated from a rat genomic library, and its sequence was determined. The small gene (.apprxeq. 850 base pairs) predicts a mRNA of .apprxeq. 500 bases [without the poly(A) tail]. The exon-intron organization is similar to that of the vasopressin gene, with 2 splice sites in the protein-coding region. The 1st exon (A) comprises the 5'' noncoding promoter region, a putative signal peptide, the nonapeptide hormone oxytocin, and the NH2-terminal, variable region of neurophysin. The 2nd exon (B) encodes the central, conserved region of neurophysin, and the 3rd exon (C) encodes the remaining COOH terminus of neurophysin, with an additional arginine residue at its end, presumably cleaved off during post-translational processing. A stretch of 143 nucleotides within exon B, except for a single base change, is entirely homologous to the equivalent part of the rat vasopressin gene, offering support for a gene conversion event having recently affected the 2 genes.