Use of Mitochondrial Cytochrome Oxidase I Polymerase Chain Reaction-Restriction Fragment Length Polymorphism for Identifying Subclades of Bemisia tabaci Mediterranean Group
Use of Mitochondrial Cytochrome Oxidase I Polymerase Chain Reaction-Restriction Fragment Length Polymorphism for Identifying Subclades of Bemisia tabaci Mediterranean Group
复制标题
DOI：
10.1603/ec11039
复制
发表时间：
2012-02-01
影响因子：
2.2
通讯作者：
Li, Xianchun
中科院分区：
文献类型：
--
作者：
Chu, Dong;Hu, Xiangshun;Li, Xianchun
甘薯粉虱的地中海群(俗称Q生物型；以下简称MED)起源于地中海地区，但目前已在地中海以外的至少10个国家发现。其中一些国家的烟粉虱标本表现出不同的害虫行为和抗药性特征，但都可能被归类为MED。对亚利桑那州收集的120个MED粉虱的线粒体细胞色素氧化酶I(MtCOI)序列(JN966761-JN966880)以及从GenBank数据库检索到的417个序列进行的系统发育分析表明，MED分为五个亚类，命名为Q1-Q5。在美国只检测到了Q1和Q2亚类。Q1和其他四个亚纲(Q2，Q5)在Alui识别位点的数量或位置上存在差异。根据本文报道的ALUI识别位点的差异和以前报道的VspI识别位点的差异，我们开发了一种简单的诊断技术，通过mtCOI聚合酶链式反应(PCR)-限制性片段长度多态性(RFLP)来识别Q1-Q5亚类。对世界各地收集的粉虱的测试表明，这种结合的mtCOI PCR-RFLP技术不仅可以可靠地将MED与中东-小亚细亚1组区分开来，而且可以将Q1与其他四个MED亚纲中的任何一个区分开来。
The Mediterranean group (commonly known as Q biotype; hereafter MED) of the sweetpotato whitefly, Bemisia tabaci (Gennadius), originated in the Mediterranean region, but it now has been found in at least 10 countries outside the Mediterranean. Collections of B. tabaci from some of these countries exhibit different pest behaviors and pesticide resistance characteristics, yet all may be classified as MED. A phylogenetic analysis of 120 mitochondrial cytochrome oxidase I (mtCOI) sequences (JN966761-JN966880) of MED whiteflies collected in Arizona and of 417 retrieved from the GenBank database resolves the MED into five subclades, designated as Q1-Q5. Only subclades Q1 and Q2 have been detected in the United States. Q1 and the other four subclades (Q2 Q5) differ in the number or position of the AluI recognition sites. Based on the differences in the AluI recognition sites reported here and the previously reported differences in VspI recognition sites, we developed a simple diagnostic technique to identify subclades Q1-Q5 by using mtCOI polymerase chain reaction (PCR)-restriction fragment-length polymorphism (RFLP). A test of a worldwide collection of whiteflies demonstrates that this combination mtCOI PCR-RFLP technique can reliably distinguish not only the MED from the Middle East-Asia Minor 1 group but also the Q1 from any of the other four MED subclades.