Characterization of Newborn Interneurons in the Mouse Olfactory Bulb Using Postnatal Electroporation
Characterization of Newborn Interneurons in the Mouse Olfactory Bulb Using Postnatal Electroporation
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使用出生后电穿孔表征小鼠嗅球中的新生中间神经元
DOI：
10.1007/978-1-4939-2459-2_7
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
A. Tsuboi
中科院分区：
文献类型：
--
作者：
Hiroo Takahashi;Sei;Ryo Asahina;Yoshiki Tamada;A. Tsuboi
感觉经验调节各种大脑结构的发育，包括皮质、海马体和嗅球(OB)。在OB中，感觉经验对神经元发育的调节在二尖瓣和簇生细胞中得到了很好的研究，但对于OB中间神经元(如颗粒细胞和球周细胞)中依赖神经元活动的形态变化知之甚少。OB中间神经元是研究出生后外界感觉输入对神经回路的调节作用的有用模型。有趣的是，OB中间神经元即使在成年阶段也会在啮齿动物身上产生并整合到先前存在的神经回路中。因此，一种有效的基因转移到新生神经元的方法将使人们更好地了解OB区的成年神经发生。在这一章中，我们描述了一种在体内将DNA电穿孔到出生后小鼠的OB中间神经元中的基本方法，而不需要复杂的手术。侧脑室微量注射质粒DNA后给予电脉冲，可有效地将基因转移到新生的OB中间神经元。利用荧光蛋白基因，标记出现在各种类型的OB中间神经元中，并持续到成年期，没有不良副作用。总体而言，出生后电穿孔是了解成人神经发生的强大工具，在时间和成本方面与其他基因操作技术相比具有优势。
Sensory experience regulates the development of various brain structures, including the cortex, hippocampus, and olfactory bulb (OB). In the OB, the regulation of the neuronal development by sensory experience is well-studied in mitral and tufted cells, but little is known about how morphological changes depending on a neuronal activity occur in OB interneurons, such as granule cells and periglomerular cells. OB interneurons are a useful model for studying the modifi cation of neural circuits by sensory inputs from the external world during postnatal stages. Interestingly, OB interneurons are generated and integrated into preexisting neural circuits in rodents even at the adult stage. Thus, an effi cient method of gene transfer into newborn neurons would allow a better understanding of adult neurogenesis in the OB. In this chapter, we describe a basic method for in vivo electroporation of DNA into OB interneurons in postnatal mice, without the need for complex surgery. Delivery of electric pulses following microinjection of plasmid DNA into the lateral ventricle enabled effi cient gene transfer into newborn OB interneurons. Utilizing a fl uorescent protein gene, labeling appears in various classes of OB interneurons and persists into adulthood with no adverse side effects. Collectively, postnatal electroporation is a powerful tool to understand adult neurogenesis, and compares favorably in terms of time and cost to other genetic manipulation techniques.
5T4糖蛋白调节嗅球中新生中间神经元特定亚型的感觉输入依赖性发育
DOI：
--
发表时间：
2012
期刊：
J . Neurosci.
影响因子：
--
作者：
Yoshihara S;Takahashi H;Naritsuka H;Nishimura N;Shirao T;Torashima T;Hirai H;Yoshihara Y;Mori K;Stern P.L. and Tsuboi A
通讯作者：
Stern P.L. and Tsuboi A
影响因子：
4.6
作者：
Yoshihara, S;Omichi, K;Yoshihara, Y
通讯作者：
Yoshihara, Y
影响因子：
16.2
作者：
Kaneko, Naoko;Marin, Oscar;Koike, Masato;Hirota, Yuki;Uchiyama, Yasuo;Wu, Jane Y.;Lu, Qiang;Tessier-Lavigne, Marc;Alvarez-Buylla, Arturo;Okano, Hideyuki;Rubenstein, John L. R.;Sawamoto, Kazunobu
通讯作者：
Sawamoto, Kazunobu