Genetic diversity among wild androecious germplasms of Diospyros kaki in China based on SSR markers
Genetic diversity among wild androecious germplasms of Diospyros kaki in China based on SSR markers
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基于SSR标记的中国野生柿子雄性种质资源遗传多样性
DOI：
10.1016/j.scienta.2018.07.020
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发表时间：
2018-12-19
影响因子：
4.3
通讯作者：
Mai, Yini
中科院分区：
文献类型：
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作者：
Wang, Liyuan;Li, Huawei;Mai, Yini
缺乏雄花严重限制了柿的受控授粉。为了解决这一问题，我们从2012年到2017年连续5年在中国收集和保存了来自各省的雄性柿资源。中国在湖北、江西、江苏、湖南、陕西、河南和广西壮族自治区共采集野生雌雄同株柿120株和雌雄同株柿10株。为了确定这些雌雄同株柿之间的遗传多样性和亲缘关系，以及中国柿与日本柿及其近缘种之间的亲缘关系，利用SSR标记对来自中国的47个野生雌雄同株柿、2个野生雌雄同株柿、10个雌雄同株柿和1个雌性柿、12个日本柿和15个近缘种进行了分析。15个座位共扩增出179个等位基因和456个等位基因，平均每个座位有11.93个等位基因和30.40个基因型。采用算术平均未加权对组法(UPGMA)将87个柿资源划分为XIII类，雌雄同株的柿与其近缘种完全区分开来。起源于同一省份的雌雄同株柿表现出较近的亲缘关系，大多数日本资源可以唯一地聚为一类。C-PCN型‘宝华甜柿’与中国3个雌雄同株柿(“麻城野师1号”、“站房后雄”和“青花雄”)和2个雌雄同株授粉恒定收敛(PCA)型(‘向阳牛心柿’和“老尖山5号”)亲缘关系密切。这些结果为有效保存和利用雌雄同株柿提供了有用的信息。
A lack of male flowers strongly restricts controlled pollination in persimmons. To address this problem, we collected and conserved male persimmon resources from various provinces in China in 5 successive years, from 2012 to 2017. Approximately 120 wild androecious persimmons and 10 monoecious persimmons were collected in Hubei, Jiangxi, Jiangsu, Hunan, Shaanxi, and Henan Provinces as well as Guangxi Zhuang Autonomous Region in China. To determine the genetic diversity and relationships among these androecious persimmons, as well as the relationship among Chinese persimmons, Japanese persimmons and closely related species, 47 wild androecious, 2 wild andromonoecious, 10 monoecious, and 1 female persimmons from China, 12 Japanese persimmons and 15 closely related species, were analyzed based on simple sequence repeat (SSR) markers. A total of 179 alleles and 456 genotypes were obtained from 15 loci, with a mean of 11.93 alleles and 30.40 genotypes per locus. The 87 persimmon resources investigated in this study were divided into XIII clusters using the unweighted pair group method with arithmetic mean average (UPGMA) and the androecious persimmons were distinguished completely from their closely related species. The androecious persimmons originating from the same provinces displayed a close genetic relationship and most Japanese resources could be classified into a same cluster exclusively. A C-PCNA type called 'Baohuatianshi' was genetically closely related to three Chinese androecious persimmons ("Macheng Yeshi-1", "Zhanfanghou-male" and "Qinghua-male") and two monoecious pollination-constant astringent (PCA) type ('Xiangyang Niuxinshi' and "Laojianshan-5"). These results provide useful information for the effective conservation and utilization of androecious persimmons.