PLANT SEEDS CONTAIN SEVERAL THIOREDOXINS OF REGULAR SIZE
PLANT SEEDS CONTAIN SEVERAL THIOREDOXINS OF REGULAR SIZE
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DOI：
10.1111/j.1432-1033.1983.tb07267.x
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
FOLLMANN, H
中科院分区：
文献类型：
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作者：
BERSTERMANN, A;VOGT, K;FOLLMANN, H
硫氧还蛋白系统由几种硫氧还蛋白同工蛋白和NADPH组成：硫氧还蛋白还原酶包含在小麦和大豆种子蛋白的白蛋白-球蛋白级分中。两个小麦硫氧还蛋白I和II的CM-纤维素分离，而大豆提取物可以分解成3硫氧还蛋白I，II和III的DEAE-纤维素。将这些蛋白质纯化至表观均一性，并通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳显示具有MW Mr. 12，000典型的单一细菌和动物硫氧还蛋白。凝胶过滤运行可能产生硫氧还蛋白MW的错误估计。种子硫氧还蛋白可作为核糖核苷酸还原酶（大肠杆菌）底物。它们刺激菠菜NADP：苹果酸脱氢酶，但对叶绿体果糖二磷酸酶无活性。非绿色植物组织中硫氧还蛋白的数量接近叶片的数量;因此，除了光依赖性和光不依赖性功能的多样化之外，还必须寻求对植物多硫氧还蛋白谱的其他解释。
Thioredoxin systems composed of several thioredoxin isoproteins and a NADPH: thioredoxin reductase are contained in the albumin-globulin fraction of wheat and soybean seed proteins. Two wheat thioredoxins I and II were separated on CM-cellulose whereas soy-bean extracts could be resolved into 3 thioredoxins I, II and III on DEAE-cellulose. These proteins were purified to apparent homogeneity and were shown by sodium dodecylsulfate/polyacrylamide gel electrophoresis to have MW Mr .apprxeq. 12,000 typical of the single bacterial and animal thioredoxin. Gel filtration runs may yield erroneous estimates of thioredoxin MW. The seed thioredoxins can serve as ribonucleotide reductase (Escherichia coli) substrates. They stimulate spinach NADP:malate dehydrogenase but are inactive towards chloroplast fructose-bisphosphatase. The number of thioredoxins in nongreen plant tissues approaches that of leaves; additional explanations must therefore be sought for the multiple thioredoxin profiles of plants besides diversification for light-dependent and light-independent functions.