Ethanol stress stimulates the Ca2+-mediated calcineurin/Crz1 pathway in Saccharomyces cerevisiae
Ethanol stress stimulates the Ca2+-mediated calcineurin/Crz1 pathway in Saccharomyces cerevisiae
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DOI：
10.1016/j.jbiosc.2008.09.005
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发表时间：
2009-01-01
影响因子：
2.8
通讯作者：
Shimoi, Hitoshi
中科院分区：
文献类型：
--
作者：
Araki, Yoshio;Wu, Hong;Shimoi, Hitoshi
环境刺激引起应激反应，这有助于维持细胞存活。在芽殖酵母酿酒酵母中，环境因素可以激活钙调磷酸酶，一种高度保守的钙和钙调蛋白依赖性蛋白磷酸酶。钙调神经磷酸酶使转录因子Crz 1去磷酸化，导致Crz 1在细胞核中积累，并在钙调神经磷酸酶依赖性反应元件（CDRE）的控制下表达应激反应基因。乙醇是酵母糖发酵的最终产物，因此是经常遇到的酵母应激源。然而，酵母适应乙醇胁迫知之甚少。在这项研究中，我们表明，乙醇刺激钙调神经磷酸酶依赖的核定位的Crz 1和CDRE依赖的基因表达。此外，CRZ 1缺失的细胞表现出对乙醇胁迫的适应缺陷，而CRZ 1的多拷贝质粒赋予对乙醇的适应性胁迫耐受性水平增加。两者合计，结果表明，乙醇激活钙调磷酸酶/CRZ 1途径，CRZ 1是至关重要的乙醇诱导的压力下的细胞存活。(C)2008年，日本生物技术学会。All rights reserved.
Environmental stimuli elicit a stress response, which helps to maintain cell survival. In budding yeast Saccharomyces cerevisiae, environmental cues can activate calcineurin, a highly conserved Ca2+- and calmodulin-dependent protein phosphatase. Calcineurin dephosphorylates the transcription factor Crz1, leading to accumulation of Crz1 in the nuclei and expression of stress responsive genes under the control of a calcineurin-dependent response element (CDRE). Ethanol is the final product of sugar fermentation by yeast, and thus a frequently encountered yeast stressor. However, adaptation of yeast to ethanol stress is poorly understood. In this study, we show that ethanol stimulates calcineurin-dependent nuclear localization of Crz1 and CDRE-dependent gene expression. Moreover, cells in which CRZ1 is deleted exhibit defective adaptation to ethanol stress, while a multicopy plasmid of CRZ1 confers an increased level of adaptive stress tolerance to ethanol. Taken together, the results indicate that ethanol activates the calcineurin/Crz1 pathway and that CRZ1 is crucial for cell survival under ethanol-induced stress. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.