RNAi-Induced Electrophysiological and Behavioral Changes Reveal Two Pheromone Binding Proteins of Helicoverpa armigera Involved in the Perception of the Main Sex Pheromone Component Z11-16:Ald
RNAi-Induced Electrophysiological and Behavioral Changes Reveal Two Pheromone Binding Proteins of Helicoverpa armigera Involved in the Perception of the Main Sex Pheromone Component Z11-16:Ald
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RNAi 诱导的电生理和行为变化揭示了棉铃虫的两种信息素结合蛋白参与主要性信息素成分 Z11-16:Ald 的感知
DOI：
10.1007/s10886-016-0816-6
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发表时间：
2017-02-01
影响因子：
2.3
通讯作者：
Zhang, Yong-Jun
中科院分区：
文献类型：
--
作者：
Dong, Kun;Sun, Liang;Zhang, Yong-Jun
信息素结合蛋白（PBP）被认为在昆虫性信息素识别中发挥关键作用；然而，与丰富的体外生化数据相比，几乎没有体内证据支持这一观点。本研究选取严重农业害虫棉铃虫的两个夜行动物PBP基因HarmPBP1和HarmPBP2进行RNA干扰敲除，然后记录雄性突变体对其主要性信息素成分(Z)-11-十六碳烯醛(Z11-16:Ald)的电生理和行为反应的变化。当任一单个 PBP 基因被敲除时，测试的雄蛾对 Z11-16: Ald 的反应没有显着的电生理或行为变化。然而，当 HarmPBP1 和 HarmPBP2 基因均被沉默时，观察到雄蛾对 Z11-16: Ald 的敏感性降低。这些结果表明，HarmPBP1 和 HarmPBP2 都是识别棉铃虫中主要性信息素成分 Z11-16：Ald 所必需的。此外，这些发现可能有助于阐明蛾 PBP 在性信息素识别途径中的生理作用，进而可以通过探索性信息素阻断剂来促进害虫控制。
Pheromone binding proteins (PBPs) are thought to play key roles in insect sex pheromone recognition; however, there is little in vivo evidence to support this viewpoint in comparison to abundant biochemical data in vitro. In the present study, two noctuid PBP genes HarmPBP1 and HarmPBP2 of the serious agricultural pest, Helicoverpa armigera were selected to be knocked down by RNA interference, and then the changes in electrophysiological and behavioral responses of male mutants to their major sex pheromone component (Z)-11-hexadecenal (Z11-16:Ald) were recorded. There were no significant electrophysiological or behavioral changes of tested male moths in response to Z11-16: Ald when either single PBP gene was knocked down. However, decreased sensitivity of male moths in response to Z11-16: Ald was observed when both HarmPBP1 and HarmPBP2 genes were silenced. These results reveal that both HarmPBP1 and HarmPBP2 are required for the recognition of the main sex pheromone component Z11-16: Ald in H. armigera. Furthermore, these findings may help clarify physiological roles of moth PBPs in the sex pheromone recognition pathway, which in turn could facilitate pest control by exploring sex pheromone blocking agents.