Myticofensin, a novel antimicrobial peptide family identified from Mytilus coruscus.
Myticofensin, a novel antimicrobial peptide family identified from Mytilus coruscus.
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DOI：
10.1016/j.fsi.2022.10.057
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发表时间：
2022-10
影响因子：
4.7
通讯作者：
Lu Liu;Menglan He;Z. Yang;Haodong Wang-;Xiao-lin Zhang;Jian-yu He;Buttino Isabella;P. Qi;Xiaojun Yan;Z. Liao
中科院分区：
文献类型：
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作者：
Lu Liu;Menglan He;Z. Yang;Haodong Wang-;Xiao-lin Zhang;Jian-yu He;Buttino Isabella;P. Qi;Xiaojun Yan;Z. Liao
在这项研究中，七个转录本代表一种新的抗菌肽（AMP）家族的结构特征相似的节肢动物防御素被确定从贻贝。这些来自贻贝AMP家族的新型防御素被命名为贻贝芬新素。为了探索这些myticofensin可能的免疫相关功能，我们研究了它们在不同组织和幼虫阶段的表达谱，以及在不同微生物威胁下的三种免疫相关组织中的表达谱。我们的数据显示，七个myticofensin在免疫相关组织中具有相对较高的表达水平。大多数myticofensin检测不到，或有低表达水平，在不同的幼虫贻贝阶段。此外，在体内微生物的挑战显着增加的表达水平的myticofensin在M。红细胞、鳃和消化腺，在不同微生物的挑战下表现出不同的免疫反应模式。我们的数据表明，不同的Myticofensin可能在不同的组织中具有不同的免疫功能。此外，合成了对应于Myticofensin的β-发夹、α-螺旋和N-末端环的肽序列，并测试了这些肽片段的抗微生物活性。我们的数据证实了贻贝防御素的多样性，并报道了这些防御素在贻贝免疫应答中对免疫相关组织中不同微生物的复杂调节。贻贝的免疫系统已经被研究了多年，因为它们是一种具有强烈环境适应性的物种。我们的数据可以被看作是一个进步的适应性研究Mytilusspp。进化的微生物世界。
In this study, seven transcripts representing a novel antimicrobial peptide (AMP) family with structural features similar to those of arthropod defensins were identified from Mytiluscoruscus. These novel defensins from the Mytilus AMP family were named myticofensins. To explore the possible immune-related functions of these myticofensins, we examined their expression profiles in different tissues and larval stages, as well as in three immune-related tissues under the threat of different microbes. Our data revealed that the seven myticofensins had relatively high expression levels in immune-related tissues. Most myticofensins were undetectable, or had low expression levels, in different larval mussel stages. Additionally,in vivomicrobial challenges significantly increased the expression levels of myticofensins inM. coruscushemocytes, gills, and digestive glands, showing different immune response patterns under challenges from different microbes. Our data indicates that different myticofensins may have different immune functions in different tissues. Furthermore, peptide sequences corresponding to the beta-hairpin, alpha-helix, and N-terminal loop of myticofensin were synthesized and the antimicrobial activities of these peptide fragments were tested. Our data confirms the diversity of defensins inMytilusand reports the complex regulation of these defensins in the mussel immune response to different microbes in immune-related tissues. The immune system ofMytilushas been studied for years as they are a species with strong environmental adaptations. Our data can be regarded as a step forward in the study of the adaptation ofMytilusspp. to an evolving microbial world.