Concentration of oxygen in lipid bilayers using a spin-label method.
Concentration of oxygen in lipid bilayers using a spin-label method.
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DOI：
10.1016/s0006-3495(83)84439-7
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发表时间：
1983-03
影响因子：
3.4
通讯作者：
W. Subczynski;J. S. Hyde
中科院分区：
文献类型：
--
作者：
W. Subczynski;J. S. Hyde
使用新型电子自旋共振（ESR）硝基氧自由基自旋探针方法测定了脂质双层烃区域中的氧浓度。对于二肉豆蔻酰磷脂酰胆碱 (DMPC)，在主转变温度以上的分配系数约为 3。在预转变温度以下迅速降至 0.2，表明结晶相中排除了氧。已确定了每个相的水和脂质之间的摩尔自由能、焓和混合熵的差异。
The concentration of oxygen in the hydrocarbon region of lipid bilayer has been determined using a novel electron spin resonance (ESR) nitroxide-radical spin-probe method. For dimyristoylphosphatidylcholine (DMPC), the partition coefficient above the main transition temperature is approximately 3. Rapid decrease to 0.2 occurs below the pretransition temperature indicating exclusion of oxygen in the crystalline phase. The differences of molar free energy, enthalpy, and entropy of mixing between water and lipid have been determined for each phase.