Reishi mushroom Ganoderma lucidum Modulates IgA production and alpha-defensin expression in the rat small intestine
Reishi mushroom Ganoderma lucidum Modulates IgA production and alpha-defensin expression in the rat small intestine
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DOI：
10.1016/j.jep.2017.12.010
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发表时间：
2018-03-25
影响因子：
5.4
通讯作者：
Iseki, Ken
中科院分区：
文献类型：
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作者：
Kubota, Atsuhito;Kobayashi, Masaki;Iseki, Ken
民族药理学相关性：免疫球蛋白A(IgA)的分泌和α-防御素在先天免疫系统中发挥作用，以防止感染。灵芝(W.Curt.：Fr.)P·卡斯特。灵芝(Reishi)是一种著名的中药材蘑菇。本研究旨在观察热石对大鼠小肠Peyer‘s patch(PP)细胞分泌IgA及小肠组织中α-防御素-5(RD-5)、RD-6表达的影响。材料与方法：大鼠口服热石粉剂0.5~5 mg/kg(1mL/kg)。给药后24小时处死所有动物。我们通过实时定量聚合酶链式反应分析检测了空肠、回肠和Peyer‘s patches(PP)中RD-5、RD-6和Toll样受体(TLR)4的mRNA水平。结果：热石可促进脂多糖(LPS)刺激PP细胞分泌IgA，增加TLR4基因的表达，但对PP细胞的活力无明显影响。热石对回肠RD-5、RD-6和TLR4基因表达的影响呈浓度依赖性。结论：热石可通过TLR4依赖途径诱导大鼠小肠IgA分泌，增加RD-5和RD-6的基因表达。目前的结果表明热石可能降低肠道感染的风险。
Ethnopharmacological relevance: Immunoglobulin A (IgA) secretion and alpha-defensins play a role in the innate immune system to protect against infection. Ganoderma lucidum (W.Curt.: Fr.) P. Karst. (Reishi) is a well-known mushroom in traditional Chinese medicine. This study aimed to determine the effects of Reishi on IgA secretion from Peyer's patch (PP) cells and alpha-defensin-5 (RD-5) and RD-6 expression in the rat small intestine.Materials and methods: The rats received an oral injection of 0.5-5 mg/kg of Reishi powder (1 mL/kg) by sonde. All animals were euthanized 24 h after Reishi administration. We examined RD-5, RD-6, and Toll-like receptor (TLR) 4 mRNA levels in the jejunum, ileum, and in Peyer's patches (PP) through quantitative real-time PCR analysis. IgA secretion from PP was measured through enzyme-linked immunosorbent assay of the supernatant after primary culture.Results: Reishi increased IgA secretion in the presence of lipopolysaccharide (LPS) and increased TLR4 mRNA levels, but had no effect on the viability of PP cells. Moreover, Reishi increased RD-5, RD-6, and TLR4 mRNA levels significantly in the ileum in a concentration-dependent manner.Conclusions: Reishi can induce IgA secretion and increase the mRNA levels of RD-5 and RD-6 in the rat small intestine, through a TLR4-dependent pathway. The present results indicate that Reishi might reduce the risk of intestinal infection.