Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration.
Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration.
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DOI：
10.1002/0471143030.cb1017s47
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发表时间：
2010-06
影响因子：
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通讯作者：
Keely, Patricia J
中科院分区：
文献类型：
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作者：
Provenzano, Paolo P;Eliceiri, Kevin W;Inman, David R;Keely, Patricia J
细胞侵入需要细胞在体内导航复杂的三维基质。提供拓扑线索，并通过肿瘤近端胶原纤维的对齐促进三维细胞迁移和侵袭。为了更好地理解细胞识别并沿沿着排列的基质迁移的分子机制，需要体外测定来概括体内基质的拓扑特征。在这里，我们描述了两种用于创建对齐的三维胶原基质的方法，这两种方法都依赖于和独立于细胞介导的对齐。包括通过二次谐波产生量化胶原基质相对于细胞的对齐和可视化的方法。这些分析很容易适用于各种细胞和与三维细胞迁移相关的生物学问题。方案Cell Biol. 47：10.17.1 - 10.17.11.© 2010年由约翰威利父子公司。
Cell invasion requires that cells navigate complex three‐dimensional matrices in vivo. Topological cues are provided and three‐dimensional cell migration and invasion facilitated by the alignment of collagen fibers proximal to tumors. In order to better understand the molecular mechanisms by which cells recognize and migrate along aligned matrices, in vitro assays are needed that recapitulate topological features of the in vivo matrix. Here, we describe two approaches for creating aligned three‐dimensional collagen matrices, both dependent and independent of cell‐mediated alignment. Approaches to quantify alignment and visualize the collagen matrix relative to the cells by second‐harmonic generation are included. These assays are readily adaptable to a variety of cells and biological questions related to three‐dimensional cell migration.Curr. Protoc. Cell Biol. 47:10.17.1‐10.17.11. © 2010 by John Wiley & Sons, Inc.